Construction of a novel vector for the nuclear transformation of the unicellular green alga Chlamydomonas reinhardtii and its stable expression

Construction of a novel vector for the nuclear transformation of the unicellular green alga Chlamydomonas reinhardtii and its stable expression

Plasmid pBI221aadAGUS which carried both of GUS (β-glucuronidase) and aadA (aminoglyoside transferase) genes besides of the 35S cauliflower mosaic virus promoter was constructed and used for stable nuclear transformation of Chlamydomonas reinhardtii. The vector was transformed into the alga by parti...

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Bibliographic Details
Main Authors: Mostafa M. EL-Sheekh, Adel W. Almutairi, Hussein E. Touliabah
Format: Article
Language:English
Published: Taylor & Francis Group 2019-12-01
Series:Journal of Taibah University for Science
Subjects:
algae
transformation
plasmid
gus gene
chlamydomonas
particle gun bombardment
Online Access:http://dx.doi.org/10.1080/16583655.2019.1603574

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