Development of monoclonal antibody and diagnostic test for Middle East respiratory syndrome coronavirus using cell-free synthesized nucleocapsid antigen

Development of monoclonal antibody and diagnostic test for Middle East respiratory syndrome coronavirus using cell-free synthesized nucleocapsid antigen

Protein nativity is one of the most critical factors for the quality of antigens used as immunogens and the reactivities of the resultant antibodies. The preparation and purification of native viral antigens in conventional cell-based protein expression systems are often accompanied by technical har...

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Bibliographic Details
Main Authors: Yutaro eYamaoka, Shutoku eMatsuyama, Shuetsu eFukushi, Satoko eMatsunaga, Yuki eMatsushima, Hiroyuki eKuroyama, Hirokazu eKimura, Makoto eTakeda, Tomoyuki eChimuro, Akihide eRyo
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-04-01
Series:Frontiers in Microbiology
Subjects:
Nucleocapsid
Monoclonal antibody
diagnosis
detection
antigen
cell-free protein synthesis
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00509/full

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