Use of Inert Gases for the Preservation of Nuclear Blood Cells

• Main Authors: Andrey Nikolaevich Khudyakov, Tatyana Vitalyevna Polezhaeva, Oksana Olegovna Zaitseva, Marta Igorevna Sergushkina, Olga Nurzadinovna Solomina
• Format: Article
• Language: English
• Published: Instituto de Tecnologia do Paraná (Tecpar)
• Series: Brazilian Archives of Biology and Technology
• Subjects: clathrates; cryopreservation; xenon; krypton; leukocytes
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100404&lng=en&tlng=en

Abstract The subject of the study was the stability of human white blood cell membranes subject to noble gases (xenon ad krypton, 0.6 mPa) clathrate cryoanabiosis (‒80°C). A unique portable stainless steel low pressure container with a compartment for flexible plastic container was designed to ensure that the cells are saturated with gases. The samples were warmed after 1 and 30 days in a water bath (+38°C) for 35-50 sec, while the container was being tilted (2-3 times per second), until the temperature of the biological object reached +3±1°C. It was demonstrated that after 30 days of clathrate anabiosis (-80°C) over 95% (of the original number) of leukocytes remain viable, and cell membranes of 54.5±3.4% of them is resistant to trypan blue; granulocyte survival rate is 73.5±2.7%, original lipid peroxidation rate and antioxidant activity are retained. Biological object cryopreservation in noble gases environment is a promising trend in biology and medicine.