Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i>
The stingless bee, <i>Melipona fasciculata</i> Smith (Apidae, Meliponini), is a native species from Brazil. Their products have high biotechnological potential, however there are no studies about the biological activities of pollen collected by <i>M. fasciculata</i>. In this...
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doaj-ad8d3cf88cd646b4908c2275db4c93582020-11-25T01:33:10ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-09-012018451210.3390/ijms20184512ijms20184512Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i>Alberto Jorge Oliveira Lopes0Cleydlenne Costa Vasconcelos1Francisco Assis Nascimento Pereira2Rosa Helena Moraes Silva3Pedro Felipe dos Santos Queiroz4Caio Viana Fernandes5João Batista Santos Garcia6Ricardo Martins Ramos7Cláudia Quintino da Rocha8Silvia Tereza de Jesus Rodrigues Moreira Lima9Maria do Socorro de Sousa Cartágenes10Maria Nilce de Sousa Ribeiro11Experimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilLaboratory of Pharmacognosy, Department of Pharmacy, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilResearch Laboratory Information Systems, Department of Information, Environment, Health and Food Production, Federal Institute of Piauí, Teresina 64000-040, BrazilChemistry Department, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilLaboratory of Pharmacognosy, Department of Pharmacy, Federal University of Maranhão, São Luís 65080-805, BrazilThe stingless bee, <i>Melipona fasciculata</i> Smith (Apidae, Meliponini), is a native species from Brazil. Their products have high biotechnological potential, however there are no studies about the biological activities of pollen collected by <i>M. fasciculata</i>. In this context, the present study investigated the chemical composition, anti-oxidant, anti-inflammatory, and analgesic activities of hydroethanolic pollen extracts collected by <i>M. fasciculata</i> in three cities in Maranhão State, Brazil. We verified the antioxidant activity of the extracts and inhibitory activity against the cyclooxygenase enzyme using in vitro assays and in allowed to select the extract with higher efficiency to be used on in vivo assays. In these trials, the selected extract showed high anti-inflammatory activity as well as nociceptive effects at central and peripheral level, suggesting that this extract acts on inhibition of histamine release and decreased synthesis of prostaglandins and the in-silico study suggested that polyphenols and acids fatty acids in the extract may be associated with these activities. The results of the present study report the high biological potential of pollen extract and we conclude that the pollen collected by <i>M. fasciculata</i> can be considered as the object of research for new pharmacological alternatives.https://www.mdpi.com/1422-0067/20/18/4512pollenextractpainanti-inflammatorynatural productsmolecular docking |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alberto Jorge Oliveira Lopes Cleydlenne Costa Vasconcelos Francisco Assis Nascimento Pereira Rosa Helena Moraes Silva Pedro Felipe dos Santos Queiroz Caio Viana Fernandes João Batista Santos Garcia Ricardo Martins Ramos Cláudia Quintino da Rocha Silvia Tereza de Jesus Rodrigues Moreira Lima Maria do Socorro de Sousa Cartágenes Maria Nilce de Sousa Ribeiro |
spellingShingle |
Alberto Jorge Oliveira Lopes Cleydlenne Costa Vasconcelos Francisco Assis Nascimento Pereira Rosa Helena Moraes Silva Pedro Felipe dos Santos Queiroz Caio Viana Fernandes João Batista Santos Garcia Ricardo Martins Ramos Cláudia Quintino da Rocha Silvia Tereza de Jesus Rodrigues Moreira Lima Maria do Socorro de Sousa Cartágenes Maria Nilce de Sousa Ribeiro Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i> International Journal of Molecular Sciences pollen extract pain anti-inflammatory natural products molecular docking |
author_facet |
Alberto Jorge Oliveira Lopes Cleydlenne Costa Vasconcelos Francisco Assis Nascimento Pereira Rosa Helena Moraes Silva Pedro Felipe dos Santos Queiroz Caio Viana Fernandes João Batista Santos Garcia Ricardo Martins Ramos Cláudia Quintino da Rocha Silvia Tereza de Jesus Rodrigues Moreira Lima Maria do Socorro de Sousa Cartágenes Maria Nilce de Sousa Ribeiro |
author_sort |
Alberto Jorge Oliveira Lopes |
title |
Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i> |
title_short |
Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i> |
title_full |
Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i> |
title_fullStr |
Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i> |
title_full_unstemmed |
Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i> |
title_sort |
anti-inflammatory and antinociceptive activity of pollen extract collected by stingless bee <i>melipona fasciculata</i> |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-09-01 |
description |
The stingless bee, <i>Melipona fasciculata</i> Smith (Apidae, Meliponini), is a native species from Brazil. Their products have high biotechnological potential, however there are no studies about the biological activities of pollen collected by <i>M. fasciculata</i>. In this context, the present study investigated the chemical composition, anti-oxidant, anti-inflammatory, and analgesic activities of hydroethanolic pollen extracts collected by <i>M. fasciculata</i> in three cities in Maranhão State, Brazil. We verified the antioxidant activity of the extracts and inhibitory activity against the cyclooxygenase enzyme using in vitro assays and in allowed to select the extract with higher efficiency to be used on in vivo assays. In these trials, the selected extract showed high anti-inflammatory activity as well as nociceptive effects at central and peripheral level, suggesting that this extract acts on inhibition of histamine release and decreased synthesis of prostaglandins and the in-silico study suggested that polyphenols and acids fatty acids in the extract may be associated with these activities. The results of the present study report the high biological potential of pollen extract and we conclude that the pollen collected by <i>M. fasciculata</i> can be considered as the object of research for new pharmacological alternatives. |
topic |
pollen extract pain anti-inflammatory natural products molecular docking |
url |
https://www.mdpi.com/1422-0067/20/18/4512 |
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