Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i>

The stingless bee, <i>Melipona fasciculata</i> Smith (Apidae, Meliponini), is a native species from Brazil. Their products have high biotechnological potential, however there are no studies about the biological activities of pollen collected by <i>M. fasciculata</i>. In this...

Full description

Bibliographic Details
Main Authors: Alberto Jorge Oliveira Lopes, Cleydlenne Costa Vasconcelos, Francisco Assis Nascimento Pereira, Rosa Helena Moraes Silva, Pedro Felipe dos Santos Queiroz, Caio Viana Fernandes, João Batista Santos Garcia, Ricardo Martins Ramos, Cláudia Quintino da Rocha, Silvia Tereza de Jesus Rodrigues Moreira Lima, Maria do Socorro de Sousa Cartágenes, Maria Nilce de Sousa Ribeiro
Format: Article
Language:English
Published: MDPI AG 2019-09-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/20/18/4512
id doaj-ad8d3cf88cd646b4908c2275db4c9358
record_format Article
spelling doaj-ad8d3cf88cd646b4908c2275db4c93582020-11-25T01:33:10ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-09-012018451210.3390/ijms20184512ijms20184512Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i>Alberto Jorge Oliveira Lopes0Cleydlenne Costa Vasconcelos1Francisco Assis Nascimento Pereira2Rosa Helena Moraes Silva3Pedro Felipe dos Santos Queiroz4Caio Viana Fernandes5João Batista Santos Garcia6Ricardo Martins Ramos7Cláudia Quintino da Rocha8Silvia Tereza de Jesus Rodrigues Moreira Lima9Maria do Socorro de Sousa Cartágenes10Maria Nilce de Sousa Ribeiro11Experimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilLaboratory of Pharmacognosy, Department of Pharmacy, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilResearch Laboratory Information Systems, Department of Information, Environment, Health and Food Production, Federal Institute of Piauí, Teresina 64000-040, BrazilChemistry Department, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilExperimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, BrazilLaboratory of Pharmacognosy, Department of Pharmacy, Federal University of Maranhão, São Luís 65080-805, BrazilThe stingless bee, <i>Melipona fasciculata</i> Smith (Apidae, Meliponini), is a native species from Brazil. Their products have high biotechnological potential, however there are no studies about the biological activities of pollen collected by <i>M. fasciculata</i>. In this context, the present study investigated the chemical composition, anti-oxidant, anti-inflammatory, and analgesic activities of hydroethanolic pollen extracts collected by <i>M. fasciculata</i> in three cities in Maranh&#227;o State, Brazil. We verified the antioxidant activity of the extracts and inhibitory activity against the cyclooxygenase enzyme using in vitro assays and in allowed to select the extract with higher efficiency to be used on in vivo assays. In these trials, the selected extract showed high anti-inflammatory activity as well as nociceptive effects at central and peripheral level, suggesting that this extract acts on inhibition of histamine release and decreased synthesis of prostaglandins and the in-silico study suggested that polyphenols and acids fatty acids in the extract may be associated with these activities. The results of the present study report the high biological potential of pollen extract and we conclude that the pollen collected by <i>M. fasciculata</i> can be considered as the object of research for new pharmacological alternatives.https://www.mdpi.com/1422-0067/20/18/4512pollenextractpainanti-inflammatorynatural productsmolecular docking
collection DOAJ
language English
format Article
sources DOAJ
author Alberto Jorge Oliveira Lopes
Cleydlenne Costa Vasconcelos
Francisco Assis Nascimento Pereira
Rosa Helena Moraes Silva
Pedro Felipe dos Santos Queiroz
Caio Viana Fernandes
João Batista Santos Garcia
Ricardo Martins Ramos
Cláudia Quintino da Rocha
Silvia Tereza de Jesus Rodrigues Moreira Lima
Maria do Socorro de Sousa Cartágenes
Maria Nilce de Sousa Ribeiro
spellingShingle Alberto Jorge Oliveira Lopes
Cleydlenne Costa Vasconcelos
Francisco Assis Nascimento Pereira
Rosa Helena Moraes Silva
Pedro Felipe dos Santos Queiroz
Caio Viana Fernandes
João Batista Santos Garcia
Ricardo Martins Ramos
Cláudia Quintino da Rocha
Silvia Tereza de Jesus Rodrigues Moreira Lima
Maria do Socorro de Sousa Cartágenes
Maria Nilce de Sousa Ribeiro
Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i>
International Journal of Molecular Sciences
pollen
extract
pain
anti-inflammatory
natural products
molecular docking
author_facet Alberto Jorge Oliveira Lopes
Cleydlenne Costa Vasconcelos
Francisco Assis Nascimento Pereira
Rosa Helena Moraes Silva
Pedro Felipe dos Santos Queiroz
Caio Viana Fernandes
João Batista Santos Garcia
Ricardo Martins Ramos
Cláudia Quintino da Rocha
Silvia Tereza de Jesus Rodrigues Moreira Lima
Maria do Socorro de Sousa Cartágenes
Maria Nilce de Sousa Ribeiro
author_sort Alberto Jorge Oliveira Lopes
title Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i>
title_short Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i>
title_full Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i>
title_fullStr Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i>
title_full_unstemmed Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee <i>Melipona fasciculata</i>
title_sort anti-inflammatory and antinociceptive activity of pollen extract collected by stingless bee <i>melipona fasciculata</i>
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2019-09-01
description The stingless bee, <i>Melipona fasciculata</i> Smith (Apidae, Meliponini), is a native species from Brazil. Their products have high biotechnological potential, however there are no studies about the biological activities of pollen collected by <i>M. fasciculata</i>. In this context, the present study investigated the chemical composition, anti-oxidant, anti-inflammatory, and analgesic activities of hydroethanolic pollen extracts collected by <i>M. fasciculata</i> in three cities in Maranh&#227;o State, Brazil. We verified the antioxidant activity of the extracts and inhibitory activity against the cyclooxygenase enzyme using in vitro assays and in allowed to select the extract with higher efficiency to be used on in vivo assays. In these trials, the selected extract showed high anti-inflammatory activity as well as nociceptive effects at central and peripheral level, suggesting that this extract acts on inhibition of histamine release and decreased synthesis of prostaglandins and the in-silico study suggested that polyphenols and acids fatty acids in the extract may be associated with these activities. The results of the present study report the high biological potential of pollen extract and we conclude that the pollen collected by <i>M. fasciculata</i> can be considered as the object of research for new pharmacological alternatives.
topic pollen
extract
pain
anti-inflammatory
natural products
molecular docking
url https://www.mdpi.com/1422-0067/20/18/4512
work_keys_str_mv AT albertojorgeoliveiralopes antiinflammatoryandantinociceptiveactivityofpollenextractcollectedbystinglessbeeimeliponafasciculatai
AT cleydlennecostavasconcelos antiinflammatoryandantinociceptiveactivityofpollenextractcollectedbystinglessbeeimeliponafasciculatai
AT franciscoassisnascimentopereira antiinflammatoryandantinociceptiveactivityofpollenextractcollectedbystinglessbeeimeliponafasciculatai
AT rosahelenamoraessilva antiinflammatoryandantinociceptiveactivityofpollenextractcollectedbystinglessbeeimeliponafasciculatai
AT pedrofelipedossantosqueiroz antiinflammatoryandantinociceptiveactivityofpollenextractcollectedbystinglessbeeimeliponafasciculatai
AT caiovianafernandes antiinflammatoryandantinociceptiveactivityofpollenextractcollectedbystinglessbeeimeliponafasciculatai
AT joaobatistasantosgarcia antiinflammatoryandantinociceptiveactivityofpollenextractcollectedbystinglessbeeimeliponafasciculatai
AT ricardomartinsramos antiinflammatoryandantinociceptiveactivityofpollenextractcollectedbystinglessbeeimeliponafasciculatai
AT claudiaquintinodarocha antiinflammatoryandantinociceptiveactivityofpollenextractcollectedbystinglessbeeimeliponafasciculatai
AT silviaterezadejesusrodriguesmoreiralima antiinflammatoryandantinociceptiveactivityofpollenextractcollectedbystinglessbeeimeliponafasciculatai
AT mariadosocorrodesousacartagenes antiinflammatoryandantinociceptiveactivityofpollenextractcollectedbystinglessbeeimeliponafasciculatai
AT marianilcedesousaribeiro antiinflammatoryandantinociceptiveactivityofpollenextractcollectedbystinglessbeeimeliponafasciculatai
_version_ 1725078942088429568