Integration of Cell-Free Expression and Solid-State NMR to Investigate the Dynamic Properties of Different Sites of the Growth Hormone Secretagogue Receptor

Integration of Cell-Free Expression and Solid-State NMR to Investigate the Dynamic Properties of Different Sites of the Growth Hormone Secretagogue Receptor

Cell-free expression represents an attractive method to produce large quantities of selectively labeled protein for NMR applications. Here, cell-free expression was used to label specific regions of the growth hormone secretagogue receptor (GHSR) with NMR-active isotopes. The GHSR is a member of the...

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Bibliographic Details
Main Authors: Emelyne M. Pacull, Franziska Sendker, Frank Bernhard, Holger A. Scheidt, Peter Schmidt, Daniel Huster, Ulrike Krug
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-10-01
Series:Frontiers in Pharmacology
Subjects:
cell-free expression
DIPSHIFT
dynamics
G protein-coupled receptors
magic angle spinning
NMR
Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2020.562113/full

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