Molecular cloning and in-depth bioinformatics analysis of type II ribosome-inactivating protein isolated from Sambucus ebulus

Molecular cloning and in-depth bioinformatics analysis of type II ribosome-inactivating protein isolated from Sambucus ebulus

Plant ribosome–inactivating proteins (RIPs) are N–glycosidases which inhibit protein synthesis through depurination of the ribosomal RNA sequence. Type II RIPs are heterodimer proteins which can bind to cell surfaces. The cytotoxicity of these RIPs is different. Sambucus spp. are a rich source of RI...

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Main Authors: Masoumeh Rezaei-Moshaei, Ali Bandehagh, Ali Dehestani, Ali Pakdin-Parizi, Majid Golkar
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:Saudi Journal of Biological Sciences
Subjects:
Physicochemical properties
Protein modeling
Recombinant RIP
Online Access:http://www.sciencedirect.com/science/article/pii/S1319562X20300516
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spelling doaj-ad81d1c4fb124af09fcffeb16efd6b5f2020-11-25T03:05:36ZengElsevierSaudi Journal of Biological Sciences1319-562X2020-06-0127616091623Molecular cloning and in-depth bioinformatics analysis of type II ribosome-inactivating protein isolated from Sambucus ebulusMasoumeh Rezaei-Moshaei0Ali Bandehagh1Ali Dehestani2Ali Pakdin-Parizi3Majid Golkar4Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, IranDepartment of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran; Corresponding authors at: Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran (A. Pakdin-Parizi); Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran (A. Bandehagh).Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, IranGenetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran; Corresponding authors at: Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran (A. Pakdin-Parizi); Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran (A. Bandehagh).Pasteur Institute of Iran, Tehran, IranPlant ribosome–inactivating proteins (RIPs) are N–glycosidases which inhibit protein synthesis through depurination of the ribosomal RNA sequence. Type II RIPs are heterodimer proteins which can bind to cell surfaces. The cytotoxicity of these RIPs is different. Sambucus spp. are a rich source of RIP proteins with different properties. In the present study, a type II RIP was isolated from S. ebulus plant that grows widely in the north of Iran, and different bioinformatics tools were used for the evaluation of physicochemical, functional and 3D protein characteristics. The results showed significant differences among isolated RIP and other Sambucus RIP proteins. The study of these differences can not only expand our insight into the functioning mechanisms of plant RIPs but also provide information about a novel RIP protein with potential biological applications.http://www.sciencedirect.com/science/article/pii/S1319562X20300516Physicochemical propertiesProtein modelingRecombinant RIP
collection DOAJ
language English
format Article
sources DOAJ
author Masoumeh Rezaei-Moshaei
Ali Bandehagh
Ali Dehestani
Ali Pakdin-Parizi
Majid Golkar
spellingShingle Masoumeh Rezaei-Moshaei
Ali Bandehagh
Ali Dehestani
Ali Pakdin-Parizi
Majid Golkar
Molecular cloning and in-depth bioinformatics analysis of type II ribosome-inactivating protein isolated from Sambucus ebulus
Saudi Journal of Biological Sciences
Physicochemical properties
Protein modeling
Recombinant RIP
author_facet Masoumeh Rezaei-Moshaei
Ali Bandehagh
Ali Dehestani
Ali Pakdin-Parizi
Majid Golkar
author_sort Masoumeh Rezaei-Moshaei
title Molecular cloning and in-depth bioinformatics analysis of type II ribosome-inactivating protein isolated from Sambucus ebulus
title_short Molecular cloning and in-depth bioinformatics analysis of type II ribosome-inactivating protein isolated from Sambucus ebulus
title_full Molecular cloning and in-depth bioinformatics analysis of type II ribosome-inactivating protein isolated from Sambucus ebulus
title_fullStr Molecular cloning and in-depth bioinformatics analysis of type II ribosome-inactivating protein isolated from Sambucus ebulus
title_full_unstemmed Molecular cloning and in-depth bioinformatics analysis of type II ribosome-inactivating protein isolated from Sambucus ebulus
title_sort molecular cloning and in-depth bioinformatics analysis of type ii ribosome-inactivating protein isolated from sambucus ebulus
publisher Elsevier
series Saudi Journal of Biological Sciences
issn 1319-562X
publishDate 2020-06-01
description Plant ribosome–inactivating proteins (RIPs) are N–glycosidases which inhibit protein synthesis through depurination of the ribosomal RNA sequence. Type II RIPs are heterodimer proteins which can bind to cell surfaces. The cytotoxicity of these RIPs is different. Sambucus spp. are a rich source of RIP proteins with different properties. In the present study, a type II RIP was isolated from S. ebulus plant that grows widely in the north of Iran, and different bioinformatics tools were used for the evaluation of physicochemical, functional and 3D protein characteristics. The results showed significant differences among isolated RIP and other Sambucus RIP proteins. The study of these differences can not only expand our insight into the functioning mechanisms of plant RIPs but also provide information about a novel RIP protein with potential biological applications.
topic Physicochemical properties
Protein modeling
Recombinant RIP
url http://www.sciencedirect.com/science/article/pii/S1319562X20300516
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