Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4
ABSTRACTThe aim of this work was to develop and evaluate an indirect enzyme-linked immunosorbent assay (ELISA) and made a serological screening for specific antibodies to BoHV-4. Bovine serum samples were collected from different Brazilian states and evaluated for the presence of antibodies for BoHV...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2015-10-01
|
| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500725&lng=en&tlng=en |
| id |
doaj-ad6d0689d44c485c897566504ecdafa5 |
|---|---|
| record_format |
Article |
| spelling |
doaj-ad6d0689d44c485c897566504ecdafa52020-11-24T23:39:23ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242015-10-0158572573110.1590/S1516-89132015050187S1516-89132015000500725Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4Ernesto Renato KrügerTânia Regina PenhaFernanda Cristina HummelgenJorge Bacila AgottaniDemétrio RevaRobson GonçalvesVanete Thomaz-SoccolABSTRACTThe aim of this work was to develop and evaluate an indirect enzyme-linked immunosorbent assay (ELISA) and made a serological screening for specific antibodies to BoHV-4. Bovine serum samples were collected from different Brazilian states and evaluated for the presence of antibodies for BoHV-4 and BoHV-1. The serological results obtained showed that the indirect ELISA assay could be applied for the detection of specific antibodies for BoHV-4. The ELISA test allowed concluding that BoHV-4 is present in bovines in every Brazilian state from which serum samples were collected. The ELISA assay here standardized proved to be useful for the epidemiological studies and showed a positivity range from 1.8 to 66% in Brazil.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500725&lng=en&tlng=enBoHV-4serologydiagnosticcattleepidemiology |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Ernesto Renato Krüger Tânia Regina Penha Fernanda Cristina Hummelgen Jorge Bacila Agottani Demétrio Reva Robson Gonçalves Vanete Thomaz-Soccol |
| spellingShingle |
Ernesto Renato Krüger Tânia Regina Penha Fernanda Cristina Hummelgen Jorge Bacila Agottani Demétrio Reva Robson Gonçalves Vanete Thomaz-Soccol Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4 Brazilian Archives of Biology and Technology BoHV-4 serology diagnostic cattle epidemiology |
| author_facet |
Ernesto Renato Krüger Tânia Regina Penha Fernanda Cristina Hummelgen Jorge Bacila Agottani Demétrio Reva Robson Gonçalves Vanete Thomaz-Soccol |
| author_sort |
Ernesto Renato Krüger |
| title |
Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4 |
| title_short |
Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4 |
| title_full |
Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4 |
| title_fullStr |
Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4 |
| title_full_unstemmed |
Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4 |
| title_sort |
development and evaluation of an indirect elisa: serological survey to detect specific antibodies to bovine herpesvirus 4 |
| publisher |
Instituto de Tecnologia do Paraná (Tecpar) |
| series |
Brazilian Archives of Biology and Technology |
| issn |
1678-4324 |
| publishDate |
2015-10-01 |
| description |
ABSTRACTThe aim of this work was to develop and evaluate an indirect enzyme-linked immunosorbent assay (ELISA) and made a serological screening for specific antibodies to BoHV-4. Bovine serum samples were collected from different Brazilian states and evaluated for the presence of antibodies for BoHV-4 and BoHV-1. The serological results obtained showed that the indirect ELISA assay could be applied for the detection of specific antibodies for BoHV-4. The ELISA test allowed concluding that BoHV-4 is present in bovines in every Brazilian state from which serum samples were collected. The ELISA assay here standardized proved to be useful for the epidemiological studies and showed a positivity range from 1.8 to 66% in Brazil. |
| topic |
BoHV-4 serology diagnostic cattle epidemiology |
| url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500725&lng=en&tlng=en |
| work_keys_str_mv |
AT ernestorenatokruger developmentandevaluationofanindirectelisaserologicalsurveytodetectspecificantibodiestobovineherpesvirus4 AT taniareginapenha developmentandevaluationofanindirectelisaserologicalsurveytodetectspecificantibodiestobovineherpesvirus4 AT fernandacristinahummelgen developmentandevaluationofanindirectelisaserologicalsurveytodetectspecificantibodiestobovineherpesvirus4 AT jorgebacilaagottani developmentandevaluationofanindirectelisaserologicalsurveytodetectspecificantibodiestobovineherpesvirus4 AT demetrioreva developmentandevaluationofanindirectelisaserologicalsurveytodetectspecificantibodiestobovineherpesvirus4 AT robsongoncalves developmentandevaluationofanindirectelisaserologicalsurveytodetectspecificantibodiestobovineherpesvirus4 AT vanetethomazsoccol developmentandevaluationofanindirectelisaserologicalsurveytodetectspecificantibodiestobovineherpesvirus4 |
| _version_ |
1725513918095294464 |