Corneal ulcer/keratitis derived Aspergillus flavus & Aspergillus tamarii and their RAPD-PCR typing

• Main Authors: Bader Alshehri, Palanisamy Manikandan, Ahmed Abdel-hadi, Abdul Aziz Bin Dukhyil, Mohammed Alaidarous, Saeed Banawas, Raed Abdullah Alharbi, Saleh Abdullah Aloyuni, Csaba Vágvölgyi, László Kredics, Sándor Kocsubé, Esam S. Al-Malki, Rajendran Vijayakumar, Kanesan Panneer Selvam, Coimbatore Subramanian Shobana
• Format: Article
• Language: English
• Published: Elsevier 2020-04-01
• Series: Journal of King Saud University: Science
• Online Access: http://www.sciencedirect.com/science/article/pii/S1018364720300689
• ISSN: 1018-3647

The aim of the study was investigate the DNA polymorphisms of Aspergillus flavus and Aspergillus tamarii isolated from regional fungal keratitis cases based on random amplified polymorphic DNA (RAPD) analyses. Thirty-two A. flavus and six A. tamarii isolates were identified using conventional macroscopic and microscopic features. Five decamer primers were used to amplify the genomic DNA sequences of the test isolates, and 140 total RAPD patterns were generated among them. From each of the 38 isolates, a range of 15–30 RAPD types were revealed. All of these were isolated from male farmers from Tamil Nadu. In the future, RAPD analyses could be employed as a reliable and rapid technique to differentiate among keratitis isolates of fungal pathogens. Keywords: RAPD PCR, Eye infection, Aspergillus flavus, Corneal ulcer, Molecular typing, Keratitis