Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes.

Although endosomal compartments have been suggested to play a role in unconventional protein secretion, there is scarce experimental evidence for such involvement. Here we report that recycling endosomes are essential for externalization of cytoplasmic secretory protein tissue transglutaminase (tTG)...

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Main Authors: Evgeny A Zemskov, Irina Mikhailenko, Ru-Ching Hsia, Liubov Zaritskaya, Alexey M Belkin
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-04-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3083433?pdf=render
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spelling doaj-ad5212c6594344929111761f4cee43782020-11-25T01:35:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-04-0164e1941410.1371/journal.pone.0019414Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes.Evgeny A ZemskovIrina MikhailenkoRu-Ching HsiaLiubov ZaritskayaAlexey M BelkinAlthough endosomal compartments have been suggested to play a role in unconventional protein secretion, there is scarce experimental evidence for such involvement. Here we report that recycling endosomes are essential for externalization of cytoplasmic secretory protein tissue transglutaminase (tTG). The de novo synthesized cytoplasmic tTG does not follow the classical ER/Golgi-dependent secretion pathway, but is targeted to perinuclear recycling endosomes, and is delivered inside these vesicles prior to externalization. On its route to the cell surface tTG interacts with internalized β1 integrins inside the recycling endosomes and is secreted as a complex with recycled β1 integrins. Inactivation of recycling endosomes, blocking endosome fusion with the plasma membrane, or downregulation of Rab11 GTPase that controls outbound trafficking of perinuclear recycling endosomes, all abrogate tTG secretion. The initial recruitment of cytoplasmic tTG to recycling endosomes and subsequent externalization depend on its binding to phosphoinositides on endosomal membranes. These findings begin to unravel the unconventional mechanism of tTG secretion which utilizes the long loop of endosomal recycling pathway and indicate involvement of endosomal trafficking in non-classical protein secretion.http://europepmc.org/articles/PMC3083433?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Evgeny A Zemskov
Irina Mikhailenko
Ru-Ching Hsia
Liubov Zaritskaya
Alexey M Belkin
spellingShingle Evgeny A Zemskov
Irina Mikhailenko
Ru-Ching Hsia
Liubov Zaritskaya
Alexey M Belkin
Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes.
PLoS ONE
author_facet Evgeny A Zemskov
Irina Mikhailenko
Ru-Ching Hsia
Liubov Zaritskaya
Alexey M Belkin
author_sort Evgeny A Zemskov
title Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes.
title_short Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes.
title_full Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes.
title_fullStr Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes.
title_full_unstemmed Unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes.
title_sort unconventional secretion of tissue transglutaminase involves phospholipid-dependent delivery into recycling endosomes.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2011-04-01
description Although endosomal compartments have been suggested to play a role in unconventional protein secretion, there is scarce experimental evidence for such involvement. Here we report that recycling endosomes are essential for externalization of cytoplasmic secretory protein tissue transglutaminase (tTG). The de novo synthesized cytoplasmic tTG does not follow the classical ER/Golgi-dependent secretion pathway, but is targeted to perinuclear recycling endosomes, and is delivered inside these vesicles prior to externalization. On its route to the cell surface tTG interacts with internalized β1 integrins inside the recycling endosomes and is secreted as a complex with recycled β1 integrins. Inactivation of recycling endosomes, blocking endosome fusion with the plasma membrane, or downregulation of Rab11 GTPase that controls outbound trafficking of perinuclear recycling endosomes, all abrogate tTG secretion. The initial recruitment of cytoplasmic tTG to recycling endosomes and subsequent externalization depend on its binding to phosphoinositides on endosomal membranes. These findings begin to unravel the unconventional mechanism of tTG secretion which utilizes the long loop of endosomal recycling pathway and indicate involvement of endosomal trafficking in non-classical protein secretion.
url http://europepmc.org/articles/PMC3083433?pdf=render
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AT irinamikhailenko unconventionalsecretionoftissuetransglutaminaseinvolvesphospholipiddependentdeliveryintorecyclingendosomes
AT ruchinghsia unconventionalsecretionoftissuetransglutaminaseinvolvesphospholipiddependentdeliveryintorecyclingendosomes
AT liubovzaritskaya unconventionalsecretionoftissuetransglutaminaseinvolvesphospholipiddependentdeliveryintorecyclingendosomes
AT alexeymbelkin unconventionalsecretionoftissuetransglutaminaseinvolvesphospholipiddependentdeliveryintorecyclingendosomes
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