Refining the Design of Diblock Elastin-Like Polypeptides for Self-Assembly into Nanoparticles

• Main Authors: Michèle Dai, Evangelos Georgilis, Guillaume Goudounet, Bertrand Garbay, Jan Pille, Jan C. M. van Hest, Xavier Schultze, Elisabeth Garanger, Sébastien Lecommandoux
• Format: Article
• Language: English
• Published: MDPI AG 2021-05-01
• Series: Polymers
• Subjects: elastin-like polypeptides; thermoresponsiveness; self-assembly; nanoparticles; methionine oxidation
• Online Access: https://www.mdpi.com/2073-4360/13/9/1470
• ISSN: 2073-4360

Diblock copolymers based-on elastin-like polypeptide (ELP) have the potential to undergo specific phase transitions when thermally stimulated. This ability is especially suitable to form carriers, micellar structures for instance, for delivering active cargo molecules. Here, we report the design and study of an ELP diblock library based on ELP-[M₁V₃-<i>i</i>]-[I-<i>j</i>]. First, ELP-[M₁V₃-<i>i</i>]-[I-<i>j</i>] (<i>i</i> = <i>20</i>, <i>40</i>, <i>60</i>; <i>j</i> = <i>20</i>, <i>90</i>) that showed a similar self-assembly propensity (unimer-to-aggregate transition) as their related monoblocks ELP-[M₁V₃-<i>i</i>] and ELP-[I-<i>j</i>]. By selectively oxidizing methionines of ELP-[M₁V₃-<i>i</i>] within the different diblocks structures, we have been able to access a thermal phase transition with three distinct regimes (unimers, micelles, aggregates) characteristic of well-defined ELP diblocks.