Synthesis and Characterization of Highly Stabilized Polymer–Trypsin Conjugates with Autolysis Resistance
Protein digestion by trypsin has been widely used in many industrial and research applications. However, extensive use of trypsin is limited because of the rapid decrease in enzymatic activity caused by autolysis at optimal pH and temperature. To improve the enzymatic performance of trypsin, we synt...
Main Authors: | Yasushi Sasai, Hiroshi Kanno, Naoki Doi, Yukinori Yamauchi, Masayuki Kuzuya, Shin-ichi Kondo |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2016-12-01
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Series: | Catalysts |
Subjects: | |
Online Access: | http://www.mdpi.com/2073-4344/7/1/4 |
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