Angiostatin prevents IL-1β-induced down-regulation of eNOS expression by inhibiting the NF-κB cascade

This study aimed to elucidate the protective potential of angiostatin in inflamed endothelial cells in culture. We assessed the effect of angiostatin on the expression of ICAM-1 and eNOS. Angiostatin prevented IL-1β-induced down-regulation of eNOS expression, but produced no significant changes on I...

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Main Authors:	Yasushi Kawasaki, Eriko Yokobayashi, Kentaro Sakamoto, Eri Tenma, Haruka Takaki, Yuki Chiba, Tomomi Otashiro, Mai Ishihara, Sei Yonezawa, Akinori Sugiyama, Yasuhiro Natori
Format:	Article
Language:	English
Published:	Elsevier 2015-11-01
Series:	Journal of Pharmacological Sciences
Subjects:	Angiostatin Anti-inflammatory Endothelial nitric oxide synthase (eNOS)
Online Access:	http://www.sciencedirect.com/science/article/pii/S1347861315002054

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spelling	doaj-ad069e013d014c788497db2800099dce2020-11-24T22:09:18ZengElsevierJournal of Pharmacological Sciences1347-86132015-11-01129320020410.1016/j.jphs.2015.10.001Angiostatin prevents IL-1β-induced down-regulation of eNOS expression by inhibiting the NF-κB cascadeYasushi KawasakiEriko YokobayashiKentaro SakamotoEri TenmaHaruka TakakiYuki ChibaTomomi OtashiroMai IshiharaSei YonezawaAkinori SugiyamaYasuhiro NatoriThis study aimed to elucidate the protective potential of angiostatin in inflamed endothelial cells in culture. We assessed the effect of angiostatin on the expression of ICAM-1 and eNOS. Angiostatin prevented IL-1β-induced down-regulation of eNOS expression, but produced no significant changes on IL-1β-induced up-regulation of ICAM-1. We then explored the effect of angiostatin on IL-1β-mediated inflammatory signaling and found that angiostatin inhibited IL-1β-mediated nuclear translocation of NF-κB. Thus, our results suggest that angiostatin prevents IL-1β-induced down-regulation of eNOS expression via inhibition of the NF-κB cascade; this may be the anti-inflammatory mechanism of angiostatin.http://www.sciencedirect.com/science/article/pii/S1347861315002054AngiostatinAnti-inflammatoryEndothelial nitric oxide synthase (eNOS)
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Yasushi Kawasaki Eriko Yokobayashi Kentaro Sakamoto Eri Tenma Haruka Takaki Yuki Chiba Tomomi Otashiro Mai Ishihara Sei Yonezawa Akinori Sugiyama Yasuhiro Natori
spellingShingle	Yasushi Kawasaki Eriko Yokobayashi Kentaro Sakamoto Eri Tenma Haruka Takaki Yuki Chiba Tomomi Otashiro Mai Ishihara Sei Yonezawa Akinori Sugiyama Yasuhiro Natori Angiostatin prevents IL-1β-induced down-regulation of eNOS expression by inhibiting the NF-κB cascade Journal of Pharmacological Sciences Angiostatin Anti-inflammatory Endothelial nitric oxide synthase (eNOS)
author_facet	Yasushi Kawasaki Eriko Yokobayashi Kentaro Sakamoto Eri Tenma Haruka Takaki Yuki Chiba Tomomi Otashiro Mai Ishihara Sei Yonezawa Akinori Sugiyama Yasuhiro Natori
author_sort	Yasushi Kawasaki
title	Angiostatin prevents IL-1β-induced down-regulation of eNOS expression by inhibiting the NF-κB cascade
title_short	Angiostatin prevents IL-1β-induced down-regulation of eNOS expression by inhibiting the NF-κB cascade
title_full	Angiostatin prevents IL-1β-induced down-regulation of eNOS expression by inhibiting the NF-κB cascade
title_fullStr	Angiostatin prevents IL-1β-induced down-regulation of eNOS expression by inhibiting the NF-κB cascade
title_full_unstemmed	Angiostatin prevents IL-1β-induced down-regulation of eNOS expression by inhibiting the NF-κB cascade
title_sort	angiostatin prevents il-1β-induced down-regulation of enos expression by inhibiting the nf-κb cascade
publisher	Elsevier
series	Journal of Pharmacological Sciences
issn	1347-8613
publishDate	2015-11-01
description	This study aimed to elucidate the protective potential of angiostatin in inflamed endothelial cells in culture. We assessed the effect of angiostatin on the expression of ICAM-1 and eNOS. Angiostatin prevented IL-1β-induced down-regulation of eNOS expression, but produced no significant changes on IL-1β-induced up-regulation of ICAM-1. We then explored the effect of angiostatin on IL-1β-mediated inflammatory signaling and found that angiostatin inhibited IL-1β-mediated nuclear translocation of NF-κB. Thus, our results suggest that angiostatin prevents IL-1β-induced down-regulation of eNOS expression via inhibition of the NF-κB cascade; this may be the anti-inflammatory mechanism of angiostatin.
topic	Angiostatin Anti-inflammatory Endothelial nitric oxide synthase (eNOS)
url	http://www.sciencedirect.com/science/article/pii/S1347861315002054
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Angiostatin prevents IL-1β-induced down-regulation of eNOS expression by inhibiting the NF-κB cascade

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