smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods

smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods

Here, the authors combine single-molecule inexpensive FISH (smiFISH) with cell membrane immunofluorescence and mathematical methods to enable whole embryo segmentation in 3D image stacks and mRNA quantification of multiple genes in each cell of the embryo.

Bibliographic Details
Main Authors: Llilians Calvo, Matthew Ronshaugen, Tom Pettini
Format: Article
Language:English
Published: Nature Publishing Group 2021-03-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-021-01803-0
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spelling doaj-acfc07cd23d04e13bfe55c8b2d03240f2021-03-21T12:28:57ZengNature Publishing GroupCommunications Biology2399-36422021-03-014111210.1038/s42003-021-01803-0smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropodsLlilians Calvo0Matthew Ronshaugen1Tom Pettini2Faculty of Biology, Medicine and Health, The University of ManchesterFaculty of Biology, Medicine and Health, The University of ManchesterFaculty of Biology, Medicine and Health, The University of ManchesterHere, the authors combine single-molecule inexpensive FISH (smiFISH) with cell membrane immunofluorescence and mathematical methods to enable whole embryo segmentation in 3D image stacks and mRNA quantification of multiple genes in each cell of the embryo.https://doi.org/10.1038/s42003-021-01803-0
collection DOAJ
language English
format Article
sources DOAJ
author Llilians Calvo
Matthew Ronshaugen
Tom Pettini
spellingShingle Llilians Calvo
Matthew Ronshaugen
Tom Pettini
smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods
Communications Biology
author_facet Llilians Calvo
Matthew Ronshaugen
Tom Pettini
author_sort Llilians Calvo
title smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods
title_short smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods
title_full smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods
title_fullStr smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods
title_full_unstemmed smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods
title_sort smifish and embryo segmentation for single-cell multi-gene rna quantification in arthropods
publisher Nature Publishing Group
series Communications Biology
issn 2399-3642
publishDate 2021-03-01
description Here, the authors combine single-molecule inexpensive FISH (smiFISH) with cell membrane immunofluorescence and mathematical methods to enable whole embryo segmentation in 3D image stacks and mRNA quantification of multiple genes in each cell of the embryo.
url https://doi.org/10.1038/s42003-021-01803-0
work_keys_str_mv AT llilianscalvo smifishandembryosegmentationforsinglecellmultigenernaquantificationinarthropods
AT matthewronshaugen smifishandembryosegmentationforsinglecellmultigenernaquantificationinarthropods
AT tompettini smifishandembryosegmentationforsinglecellmultigenernaquantificationinarthropods
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