smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods

smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods

Here, the authors combine single-molecule inexpensive FISH (smiFISH) with cell membrane immunofluorescence and mathematical methods to enable whole embryo segmentation in 3D image stacks and mRNA quantification of multiple genes in each cell of the embryo.

Bibliographic Details
Main Authors: Llilians Calvo, Matthew Ronshaugen, Tom Pettini
Format: Article
Language:English
Published: Nature Publishing Group 2021-03-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-021-01803-0
Description
Summary:Here, the authors combine single-molecule inexpensive FISH (smiFISH) with cell membrane immunofluorescence and mathematical methods to enable whole embryo segmentation in 3D image stacks and mRNA quantification of multiple genes in each cell of the embryo.
ISSN:2399-3642

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