Evaluation of Measuring Radiation-Induced Apoptosis in Human T-Lymphocytes by Flow Cytometry as a Biological Dosimetry System

• Main Authors: Mohammad Bagher Tavakkoli, Majid Kheirollahi, Ali Kiani, Mohammad Kazemi, Leili Mohebat, Shagayegh Haghjooy Javanmard, Mahnaz Roayaei
• Format: Article
• Language: fas
• Published: Vesnu Publications 2014-07-01
• Series: مجله دانشکده پزشکی اصفهان
• Subjects: Apoptosis; Flowcytometry; T-cells; Lymphocyte; Biological dosimetry; Radiation Apoptosis; Radiation
• Online Access: http://jims.mui.ac.ir/index.php/jims/article/view/3810
• ISSN: 1027-7595; 1735-854X

Background: In events such as a nuclear explosion or leakage of radioactive material from nuclear power dungeons or other events in nuclear medicine departments in hospitals, many people accidentally receive an unspecified amount of ionizing radiation. First step to treat is evaluation of radiation dose received by the victims. In such situation and in radiotherapy program, dosimetry is used for evaluation of treatment planning. Some measurable biological indexes used for evaluation of dose of radiation. Some measurable biological indexes can be used in biological dosimetry to measure the radiation dose and estimate the radiation effect. Methods: In this study, the test for biological dosimetry based on apoptosis induced by gamma radiation in peripheral blood T-lymphocytes was performed in 16 volunteers. The blood lymphocytes were isolated and cultured in RPMI (Roswell Park Memorial Institute) 1640 medium and then, were placed in 5% CO2 atmosphere at 37°C. Then, the samples were prepared from the culture medium and radiated with different doses of gamma radiation. Sample transferred to incubator again to measure their apoptosis. Radiation-induced apoptosis in the cell population was measured by flow cytometry using Annexin V + fluorescein isothiocyanate (FITC) and prodidium iodide (PI) stains. Findings: Radiation induced apoptosis was measureable with enough precision. But measured apoptosis depended on delay time after irradiation and protocole of flow cytometry. Conclusion: The results of this study show that it is possible to use radiation for measuring apoptosis as a biological dosimeter in a short time after radiation exposure in the events such as a nuclear explosion or leakage of radioactive material.