Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase
Flaviviruses use a ~70 nucleotide stem-loop structure called stem-loop A (SLA) at the 5’ end of the RNA genome as a promoter for RNA synthesis by the viral polymerase NS5. Here the authors describe the structures of dengue and Zika virus SLAs, identify the SLA-binding site on NS5, and propose models...
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2021-05-01
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Online Access: | https://doi.org/10.1038/s41467-021-22846-1 |
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doaj-acf8de90f3514ec8ab19ff937e51ff752021-05-09T11:13:01ZengNature Publishing GroupNature Communications2041-17232021-05-0112111210.1038/s41467-021-22846-1Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymeraseEunhye Lee0Paul J. Bujalowski1Tadahisa Teramoto2Keerthi Gottipati3Seth D. Scott4Radhakrishnan Padmanabhan5Kyung H. Choi6Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical BranchDepartment of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical BranchDepartment of Microbiology and Immunology, Georgetown University School of MedicineDepartment of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical BranchDepartment of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical BranchDepartment of Microbiology and Immunology, Georgetown University School of MedicineDepartment of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical BranchFlaviviruses use a ~70 nucleotide stem-loop structure called stem-loop A (SLA) at the 5’ end of the RNA genome as a promoter for RNA synthesis by the viral polymerase NS5. Here the authors describe the structures of dengue and Zika virus SLAs, identify the SLA-binding site on NS5, and propose models for how NS5 recognizes the RNA promoter.https://doi.org/10.1038/s41467-021-22846-1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eunhye Lee Paul J. Bujalowski Tadahisa Teramoto Keerthi Gottipati Seth D. Scott Radhakrishnan Padmanabhan Kyung H. Choi |
spellingShingle |
Eunhye Lee Paul J. Bujalowski Tadahisa Teramoto Keerthi Gottipati Seth D. Scott Radhakrishnan Padmanabhan Kyung H. Choi Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase Nature Communications |
author_facet |
Eunhye Lee Paul J. Bujalowski Tadahisa Teramoto Keerthi Gottipati Seth D. Scott Radhakrishnan Padmanabhan Kyung H. Choi |
author_sort |
Eunhye Lee |
title |
Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase |
title_short |
Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase |
title_full |
Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase |
title_fullStr |
Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase |
title_full_unstemmed |
Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase |
title_sort |
structures of flavivirus rna promoters suggest two binding modes with ns5 polymerase |
publisher |
Nature Publishing Group |
series |
Nature Communications |
issn |
2041-1723 |
publishDate |
2021-05-01 |
description |
Flaviviruses use a ~70 nucleotide stem-loop structure called stem-loop A (SLA) at the 5’ end of the RNA genome as a promoter for RNA synthesis by the viral polymerase NS5. Here the authors describe the structures of dengue and Zika virus SLAs, identify the SLA-binding site on NS5, and propose models for how NS5 recognizes the RNA promoter. |
url |
https://doi.org/10.1038/s41467-021-22846-1 |
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