Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase

Flaviviruses use a ~70 nucleotide stem-loop structure called stem-loop A (SLA) at the 5’ end of the RNA genome as a promoter for RNA synthesis by the viral polymerase NS5. Here the authors describe the structures of dengue and Zika virus SLAs, identify the SLA-binding site on NS5, and propose models...

Full description

Bibliographic Details
Main Authors: Eunhye Lee, Paul J. Bujalowski, Tadahisa Teramoto, Keerthi Gottipati, Seth D. Scott, Radhakrishnan Padmanabhan, Kyung H. Choi
Format: Article
Language:English
Published: Nature Publishing Group 2021-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-22846-1
id doaj-acf8de90f3514ec8ab19ff937e51ff75
record_format Article
spelling doaj-acf8de90f3514ec8ab19ff937e51ff752021-05-09T11:13:01ZengNature Publishing GroupNature Communications2041-17232021-05-0112111210.1038/s41467-021-22846-1Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymeraseEunhye Lee0Paul J. Bujalowski1Tadahisa Teramoto2Keerthi Gottipati3Seth D. Scott4Radhakrishnan Padmanabhan5Kyung H. Choi6Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical BranchDepartment of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical BranchDepartment of Microbiology and Immunology, Georgetown University School of MedicineDepartment of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical BranchDepartment of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical BranchDepartment of Microbiology and Immunology, Georgetown University School of MedicineDepartment of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical BranchFlaviviruses use a ~70 nucleotide stem-loop structure called stem-loop A (SLA) at the 5’ end of the RNA genome as a promoter for RNA synthesis by the viral polymerase NS5. Here the authors describe the structures of dengue and Zika virus SLAs, identify the SLA-binding site on NS5, and propose models for how NS5 recognizes the RNA promoter.https://doi.org/10.1038/s41467-021-22846-1
collection DOAJ
language English
format Article
sources DOAJ
author Eunhye Lee
Paul J. Bujalowski
Tadahisa Teramoto
Keerthi Gottipati
Seth D. Scott
Radhakrishnan Padmanabhan
Kyung H. Choi
spellingShingle Eunhye Lee
Paul J. Bujalowski
Tadahisa Teramoto
Keerthi Gottipati
Seth D. Scott
Radhakrishnan Padmanabhan
Kyung H. Choi
Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase
Nature Communications
author_facet Eunhye Lee
Paul J. Bujalowski
Tadahisa Teramoto
Keerthi Gottipati
Seth D. Scott
Radhakrishnan Padmanabhan
Kyung H. Choi
author_sort Eunhye Lee
title Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase
title_short Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase
title_full Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase
title_fullStr Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase
title_full_unstemmed Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase
title_sort structures of flavivirus rna promoters suggest two binding modes with ns5 polymerase
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2021-05-01
description Flaviviruses use a ~70 nucleotide stem-loop structure called stem-loop A (SLA) at the 5’ end of the RNA genome as a promoter for RNA synthesis by the viral polymerase NS5. Here the authors describe the structures of dengue and Zika virus SLAs, identify the SLA-binding site on NS5, and propose models for how NS5 recognizes the RNA promoter.
url https://doi.org/10.1038/s41467-021-22846-1
work_keys_str_mv AT eunhyelee structuresofflavivirusrnapromoterssuggesttwobindingmodeswithns5polymerase
AT pauljbujalowski structuresofflavivirusrnapromoterssuggesttwobindingmodeswithns5polymerase
AT tadahisateramoto structuresofflavivirusrnapromoterssuggesttwobindingmodeswithns5polymerase
AT keerthigottipati structuresofflavivirusrnapromoterssuggesttwobindingmodeswithns5polymerase
AT sethdscott structuresofflavivirusrnapromoterssuggesttwobindingmodeswithns5polymerase
AT radhakrishnanpadmanabhan structuresofflavivirusrnapromoterssuggesttwobindingmodeswithns5polymerase
AT kyunghchoi structuresofflavivirusrnapromoterssuggesttwobindingmodeswithns5polymerase
_version_ 1721454628332109824