Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem Cells

Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem Cells

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<p>Abstract</p> <p>The dissociation of adherent mesenchymal stem cell (MSC) monolayers with trypsin and enzyme-free dissociation buffer was compared. A significantly lower proportion of viable cells were obtained with enzyme-free dissociation buffers compared to trypsin. Subsequent...

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Bibliographic Details
Main Authors: Heng Boon, Cowan Catherine, Basu Shubhayu
Format: Article
Language:English
Published: BMC 2009-01-01
Series:Biological Procedures Online
Subjects:
Dissociation
Enzyme
Mesenchymal
Stem cells
Trypsin
Online Access:http://www.biologicalproceduresonline.com/content/11/1/161

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