Occurrence of anti-Neospora caninum antibodies in cattle in the dairy farming region of the state of Piauí, Brazil

• Main Authors: Raylson Pereira de Oliveira, Nínivy Marques Soares, José Givanildo da Silva, Müller Ribeiro-Andrade, Nair Silva Cavalcanti de Lira, Márcia Paula Oliveira Farias, Rinaldo Aparecido Mota
• Format: Article
• Language: English
• Published: Colégio Brasileiro de Parasitologia Veterinaria 2018-08-01
• Series: Revista Brasileira de Parasitologia Veterinária
• Subjects: Bovinos; neosporose; reação de imunofluorescência indireta
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018005010102&lng=en&tlng=en

Abstract This study focused on the detection of anti-Neospora caninum IgG antibodies in cows in the dairy farming region of the state of Piauí, Brazil. To this end, serum samples were collected from 255 dairy cows on 17 farms located in the dairy farming region of the municipality of Parnaíba, Piauí, Brazil. The indirect fluorescence antibody test (IFAT) was employed to detect anti-N. caninum IgG antibodies, using anti-bovine IgG (Sigma®) conjugated to fluorescein isothiocyanate (FITC) and a cutoff point of 1:200. Of the 255 samples analyzed, 69 (27.06%) were positive for anti- N. caninum IgG antibodies, the relative frequency found by property was: 1 (20.00%), 2 (53.33%), 3 (46.66%), 4 (53.33%), 5 (26.66%), 6 (6.66%), 7 (6.66%), 8 (20.00%), 9 (26.66%), 10 (26.66%), 11 (20.00%), 12 (20.00%), 13 (46.66%), 14 (26.66%), 15 (26.66%), 16 (20.00%) and 17 (13.33%). with titers of 200 (15.94%), 400 (20.30%), 800 (24.63%), 1600 (23.18%) and 3200 (15.94%), being the highest frequency for the titer of 800. This study demonstrates for the first time that cows from dairy herds of Parnaíba municipality, state of Piauí, are exposed to N. caninum.