Monitoring Protein Secretion in Streptomyces Using Fluorescent Proteins

Monitoring Protein Secretion in Streptomyces Using Fluorescent Proteins

Fluorescent proteins are a major cell biology tool to analyze protein sub-cellular topology. Here we have applied this technology to study protein secretion in the Gram-positive bacterium Streptomyces lividans TK24, a widely used host for heterologous protein secretion biotechnology. Green and monom...

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Bibliographic Details
Main Authors: Mohamed Belal Hamed, Kristof Vrancken, Bohdan Bilyk, Joachim Koepff, Renata Novakova, Lieve van Mellaert, Marco Oldiges, Andriy Luzhetskyy, Jan Kormanec, Jozef Anné, Spyridoula Karamanou, Anastassios Economou
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-12-01
Series:Frontiers in Microbiology
Subjects:
eGFP
mRFP
protein secretion
signal peptide
Streptomyces lividans
protein secretion biotechnology
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.03019/full

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