An Efficient Ligation Method in the Making of an in vitro Virus for in vitro Protein Evolution

An Efficient Ligation Method in the Making of an in vitro Virus for in vitro Protein Evolution

<p>The <it>&ldquo;in vitro</it> virus&rdquo; is a molecular construct to perform evolutionary protein engineering. The &ldquo;virion (=viral particle)&rdquo; (mRNA-peptide fusion), is made by bonding a nascent protein with its coding mRNA via puromycin in a test tub...

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Bibliographic Details
Main Authors: Tabuchi Ichiro, Soramoto Sayaka, Suzuki Miho, Nishigaki Koichi, Nemoto Naoto, Husimi Yuzuru
Format: Article
Language:English
Published: BMC 2002-01-01
Series:Biological Procedures Online
Subjects:
methods
protein engineering
Online Access:http://www.biologicalprocedures.com/bpo/arts/1/33/m33.htm

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