An Efficient Ligation Method in the Making of an in vitro Virus for in vitro Protein Evolution
<p>The <it>“in vitro</it> virus” is a molecular construct to perform evolutionary protein engineering. The “virion (=viral particle)” (mRNA-peptide fusion), is made by bonding a nascent protein with its coding mRNA via puromycin in a test tub...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2002-01-01
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Series: | Biological Procedures Online |
Subjects: | |
Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/33/m33.htm |