Evaluation of Primers Targeting the Diazotroph Functional Gene and Development of NifMAP – A Bioinformatics Pipeline for Analyzing nifH Amplicon Data

Evaluation of Primers Targeting the Diazotroph Functional Gene and Development of NifMAP – A Bioinformatics Pipeline for Analyzing nifH Amplicon Data

Diazotrophic microorganisms introduce biologically available nitrogen (N) to the global N cycle through the activity of the nitrogenase enzyme. The genetically conserved dinitrogenase reductase (nifH) gene is phylogenetically distributed across four clusters (I–IV) and is widely used as a marker gen...

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Bibliographic Details
Main Authors: Roey Angel, Maximilian Nepel, Christopher Panhölzl, Hannes Schmidt, Craig W. Herbold, Stephanie A. Eichorst, Dagmar Woebken
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-04-01
Series:Frontiers in Microbiology
Subjects:
nitrogen fixation
primer evaluation
nifH gene
Illumina amplicon sequencing
NifMAP
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2018.00703/full

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