Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction

• Main Authors: Pierre Saboural, Frédéric Chaubet, Francois Rouzet, Faisal Al-Shoukr, Rana Ben Azzouna, Nadia Bouchemal, Luc Picton, Liliane Louedec, Murielle Maire, Lydia Rolland, Guy Potier, Dominique Le Guludec, Didier Letourneur, Cédric Chauvierre
• Format: Article
• Language: English
• Published: MDPI AG 2014-09-01
• Series: Marine Drugs
• Subjects: fucoidan; molecular imaging; atherothrombosis; SPECT
• Online Access: http://www.mdpi.com/1660-3397/12/9/4851

Fucoidans constitute a large family of sulfated polysaccharides with several biochemical properties. A commercial fucoidan from brown algae, containing low molecular weight polysaccharidic species constituted of l-fucose, uronic acids and sulfate groups, was simply treated here with calcium acetate solution. This treatment led to a purified fraction with a yield of 45%. The physicochemical characterizations of the purified fucoidan using colorimetric assay, MALLS, dRI, FT-IR, NMR, exhibited molecular weight distributions and chemical profiles similar for both fucoidans whereas the sulfate and l-fucose contents increased by 16% and 71%, respectively. The biodistribution study in rat of both compounds labeled with 99mTc evidenced a predominant renal elimination of the purified fucoidan, but the crude fucoidan was mainly retained in liver and spleen. In rat myocardial ischemia-reperfusion, we then demonstrated the better efficiency of the purified fucoidan. This purified sulfated polysaccharide appears promising for the development of molecular imaging in acute coronary syndrome.