AVALIAÇÃO DA BIOMASSA DE FUNGOS AMAZÔNICOS COMO FONTE DE LIPASES PARA BIOCATÁLISE

• Main Authors: Israel P. Romano, Vanderlei S. dos Santos, Ana Carolina de Lima Paes Louzada, Raimundo C. Pereira Junior, Edson J. do Carmo, Adolfo José da Mota, Hiléia dos S. Barroso, Ivaldo Itabaiana Junior, José Odair Pereira, Spartaco Astolfi Filho, Sandra Patrícia Zanotto
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química
• Series: Química Nova
• Subjects: lipase; biocatalysis; biomass; enantioselectivity; transesterification; amazonian fungi
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422020000200146&lng=en&tlng=en
• ISSN: 0100-4042; 1678-7064

We evaluated the biomass of twenty Amazonian fungal isolates as a potential source of mycelium-bound lipases with hydrolytic, synthetic or enantioselective activity for biocatalysis application. We compared the hydrolytic activity of three biomass treatments (delipidated, non-delipidated and cultivated in medium without inducer). Delipidated biomass showed better results in the hydrolysis of p-nitrophenyl palmitate compared to the other two treatments for fifteen isolates. Delipidated biomass of six Aspergillus strains and UEA_115 strain showed a high synthesis activity of ethyl palmitate by transesterification in organic medium. Results were confirmed by spectrophotometry (410 nm) and gas chromatography. In this reaction, the isolate DPUA_1539 A. flavo-furcatis reached a maximum value of 668.5 ± 23.5 mU g-1. Enantioselective activity assays indicated that biomass-bound lipases from UEA_115 isolate (E = 3.58; ees = 7 ± 0) and in particular from the DPUA_1539 A. flavo-furcatis isolate (E = 24.15; ees = 91 ± 1) have the ability to discriminate enantiomers of the drug ibuprofen by ester synthesis, preferably with (R)-enantiomer. These results encourage further investigations of these fungi as potential lipase suppliers for biocatalytic processes such as biodiesel production and enantiopure drugs.