Comparision of Aqueous and Hydroalcoholic Extracts of Foeniculum Vulgare and Carum Copticum with Gentamicin on Escherichia Coli Strains: in Vitro Study

• Main Authors: F Abedi, R Rajabnia, H Sorkhi, Z Memariani, H Shirafkan, SA Mozaffarpur
• Format: Article
• Language: English
• Published: Babol University of Medical Sciences 2018-02-01
• Series: Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul
• Subjects: Anti-Bacterial Agents; Ajwain; Fennel; Persian Medicine
• Online Access: http://jbums.org/browse.php?a_code=A-10-3728-1&slc_lang=en&sid=1

BACKGROUND AND OBJECTIVE: Due to bacterial resistance to antibiotics, new antibacterial agents is essential. In Persian medicine Fennel (Fuenoculum vulgare Mill.) and Ajwain (Carum copticum (L.) Benth. & Hook.f.) are recommended for the treatment of some infections. In this research, bacteriostatic and bactericidal effects of aqueous and hydroalcoholic extracts of fennel and Ajwain on E. coli were investigated. METHODS: In an in-vitro study 30 clinical isolates of urine culture of children with urinary tract infection from Amirkola Pediatric Hospital in Babol and a standard sample were used. Antibacterial effects of 4 grouos including aqueous and hydroalcoholic extracts of fennel and Ajwain by measuring the diameter of the inhibition zone using disc diffusion (concentrations 16, 32, 64, 128, 256 and 512 mg/disc) and determination of Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentration (MBC) with Microdilution method was compared with Gentamicin (30mg/disc) as a positive control FINDINGS: There was no significant difference in inhibition zone with Gentamicin at concentrations of 64, 128, 256, and 512 mg/disc in standard and clinical samples. At concentrations of 16 and 32, Gentamicin was significantly better. The extract of 512 mg/disc (12.93±2.66) of hydroalcoholic extract of Carum copticum was significantly better than 256 mg/disc (9.53±1) (p=0.002). The MIC and MBC for standard samples were 4 and 8, respectively, and for clinical samples 3.83±2.36 and 5.8 mg / ml, respectively. Other extracts were not able to inhibit the growth of Escherichia coli. CONCLUSION: The results showed that the Hydroalcoholic extract of Carum copticum has bacteriostatic and bactericidal effects on standard and clinical isolates of Escherichia coli.