Generation of three miR-122 knockout lines from a human embryonic stem cell line
miR-122 is the most abundant miRNA in the human liver, accounting for 52% of the entire hepatic miRNome. Previous studies have demonstrated that miR-122 plays key roles in hepatocyte growth, metabolism, and homeostasis. Here, we created three miR-122 knockout human embryonic stem cell line lines, WA...
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Elsevier
2017-10-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506117301046 |
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doaj-ac0dca841d6544bc8a6e112e3dedb85f2020-11-24T23:39:26ZengElsevierStem Cell Research1873-50611876-77532017-10-0124C16416810.1016/j.scr.2017.06.004Generation of three miR-122 knockout lines from a human embryonic stem cell lineYanli Liu0Feima Wu1Yuanqi Zhuang2Dongsheng Guo3Nasir Abbas4Ruzi Wei5Kepin Wang6Yan Chen7Jiawang Tao8Yuhang Wu9Fang Yuan10Tingcai Pan11Fan Yang12Keyu Lai13Liangxue Lai14Yin-xiong Li15Institute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaInstitute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaInstitute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaInstitute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaInstitute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaInstitute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaKey Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaInstitute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaInstitute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaInstitute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaInstitute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaInstitute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaInstitute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaKey Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaInstitute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinamiR-122 is the most abundant miRNA in the human liver, accounting for 52% of the entire hepatic miRNome. Previous studies have demonstrated that miR-122 plays key roles in hepatocyte growth, metabolism, and homeostasis. Here, we created three miR-122 knockout human embryonic stem cell line lines, WAe001-A-7, WAe001-A-8, and WAe001-A-9, using the CRISPR/Cas9 technique. These mutated cell lines retained their pluripotency, in vitro differentiation potential, normal morphology, and karyotype.http://www.sciencedirect.com/science/article/pii/S1873506117301046 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Yanli Liu Feima Wu Yuanqi Zhuang Dongsheng Guo Nasir Abbas Ruzi Wei Kepin Wang Yan Chen Jiawang Tao Yuhang Wu Fang Yuan Tingcai Pan Fan Yang Keyu Lai Liangxue Lai Yin-xiong Li |
| spellingShingle |
Yanli Liu Feima Wu Yuanqi Zhuang Dongsheng Guo Nasir Abbas Ruzi Wei Kepin Wang Yan Chen Jiawang Tao Yuhang Wu Fang Yuan Tingcai Pan Fan Yang Keyu Lai Liangxue Lai Yin-xiong Li Generation of three miR-122 knockout lines from a human embryonic stem cell line Stem Cell Research |
| author_facet |
Yanli Liu Feima Wu Yuanqi Zhuang Dongsheng Guo Nasir Abbas Ruzi Wei Kepin Wang Yan Chen Jiawang Tao Yuhang Wu Fang Yuan Tingcai Pan Fan Yang Keyu Lai Liangxue Lai Yin-xiong Li |
| author_sort |
Yanli Liu |
| title |
Generation of three miR-122 knockout lines from a human embryonic stem cell line |
| title_short |
Generation of three miR-122 knockout lines from a human embryonic stem cell line |
| title_full |
Generation of three miR-122 knockout lines from a human embryonic stem cell line |
| title_fullStr |
Generation of three miR-122 knockout lines from a human embryonic stem cell line |
| title_full_unstemmed |
Generation of three miR-122 knockout lines from a human embryonic stem cell line |
| title_sort |
generation of three mir-122 knockout lines from a human embryonic stem cell line |
| publisher |
Elsevier |
| series |
Stem Cell Research |
| issn |
1873-5061 1876-7753 |
| publishDate |
2017-10-01 |
| description |
miR-122 is the most abundant miRNA in the human liver, accounting for 52% of the entire hepatic miRNome. Previous studies have demonstrated that miR-122 plays key roles in hepatocyte growth, metabolism, and homeostasis. Here, we created three miR-122 knockout human embryonic stem cell line lines, WAe001-A-7, WAe001-A-8, and WAe001-A-9, using the CRISPR/Cas9 technique. These mutated cell lines retained their pluripotency, in vitro differentiation potential, normal morphology, and karyotype. |
| url |
http://www.sciencedirect.com/science/article/pii/S1873506117301046 |
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