Detection of 16S Mitochondrial Gene Polymorphism on Barb Fish (Barbodes binotatus Valenciennes, 1842) from Lake Lebo Taliwang, West Nusa Tenggara

The spotted barb fish (Barbodes binotatus), a tropical cyprinid fish, is one of the abundant fishes in Lake Lebo Taliwang commonly consumed due to high nutrition and utilized as an aquarium trade due to their exotic colour. However, no genetic information related to the intra-population genetic vari...

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Main Authors: Tuty Arisuryanti, Astin Alfianti, Nadya Ulfa Nida' Firdaus, Lukman Hakim
Format: Article
Language:Indonesian
Published: Universitas Gadjah Mada 2020-12-01
Series:Jurnal Perikanan Universitas Gadjah Mada
Subjects:
Online Access:https://jurnal.ugm.ac.id/jfs/article/view/54064
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spelling doaj-aba6deddf39747f789145690109d6cd92020-12-30T09:00:17ZindUniversitas Gadjah MadaJurnal Perikanan Universitas Gadjah Mada0853-63842502-50662020-12-0122212312610.22146/jfs.5406428933Detection of 16S Mitochondrial Gene Polymorphism on Barb Fish (Barbodes binotatus Valenciennes, 1842) from Lake Lebo Taliwang, West Nusa TenggaraTuty Arisuryanti0Astin Alfianti1Nadya Ulfa Nida' Firdaus2Lukman Hakim3Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, IndonesiaLaboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, IndonesiaLaboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, IndonesiaLaboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, IndonesiaThe spotted barb fish (Barbodes binotatus), a tropical cyprinid fish, is one of the abundant fishes in Lake Lebo Taliwang commonly consumed due to high nutrition and utilized as an aquarium trade due to their exotic colour. However, no genetic information related to the intra-population genetic variation of the fish species in Lake Lebo Taliwang. Genetic information of the spotted barb fish can play an important role for the fish conservation. Therefore, the objective of this study was to detect 16S mitochondrial gene polymorphism of the spotted barb fish which can be used to detect their genetic variation. The method used in this study was a PCR method with primers 16Sar and 16Sbr. The data was then analysed using MESQUITE, MEGA, DnaSP and PopART to identify 16S mitochondrial gene polymorphism. This study obtained 610 base pairs after alignment of 16S sequences of all samples. Five haplotypes were detected with 9 variable sites and 2 parsimony informative sites. Haplotype diversity and nucleotide diversity of the spotted barb fish were 1.00 and 0.0066 respectively. In addition, genetic distance among the samples was 0.66. This finding exhibited polymorphism on 16S gene sequence among the fish samples and this data indicated genetic variation of the spotted barb from Lake Lebo Taliwang.https://jurnal.ugm.ac.id/jfs/article/view/54064barbodes binotatus16s genepolymorphism
collection DOAJ
language Indonesian
format Article
sources DOAJ
author Tuty Arisuryanti
Astin Alfianti
Nadya Ulfa Nida' Firdaus
Lukman Hakim
spellingShingle Tuty Arisuryanti
Astin Alfianti
Nadya Ulfa Nida' Firdaus
Lukman Hakim
Detection of 16S Mitochondrial Gene Polymorphism on Barb Fish (Barbodes binotatus Valenciennes, 1842) from Lake Lebo Taliwang, West Nusa Tenggara
Jurnal Perikanan Universitas Gadjah Mada
barbodes binotatus
16s gene
polymorphism
author_facet Tuty Arisuryanti
Astin Alfianti
Nadya Ulfa Nida' Firdaus
Lukman Hakim
author_sort Tuty Arisuryanti
title Detection of 16S Mitochondrial Gene Polymorphism on Barb Fish (Barbodes binotatus Valenciennes, 1842) from Lake Lebo Taliwang, West Nusa Tenggara
title_short Detection of 16S Mitochondrial Gene Polymorphism on Barb Fish (Barbodes binotatus Valenciennes, 1842) from Lake Lebo Taliwang, West Nusa Tenggara
title_full Detection of 16S Mitochondrial Gene Polymorphism on Barb Fish (Barbodes binotatus Valenciennes, 1842) from Lake Lebo Taliwang, West Nusa Tenggara
title_fullStr Detection of 16S Mitochondrial Gene Polymorphism on Barb Fish (Barbodes binotatus Valenciennes, 1842) from Lake Lebo Taliwang, West Nusa Tenggara
title_full_unstemmed Detection of 16S Mitochondrial Gene Polymorphism on Barb Fish (Barbodes binotatus Valenciennes, 1842) from Lake Lebo Taliwang, West Nusa Tenggara
title_sort detection of 16s mitochondrial gene polymorphism on barb fish (barbodes binotatus valenciennes, 1842) from lake lebo taliwang, west nusa tenggara
publisher Universitas Gadjah Mada
series Jurnal Perikanan Universitas Gadjah Mada
issn 0853-6384
2502-5066
publishDate 2020-12-01
description The spotted barb fish (Barbodes binotatus), a tropical cyprinid fish, is one of the abundant fishes in Lake Lebo Taliwang commonly consumed due to high nutrition and utilized as an aquarium trade due to their exotic colour. However, no genetic information related to the intra-population genetic variation of the fish species in Lake Lebo Taliwang. Genetic information of the spotted barb fish can play an important role for the fish conservation. Therefore, the objective of this study was to detect 16S mitochondrial gene polymorphism of the spotted barb fish which can be used to detect their genetic variation. The method used in this study was a PCR method with primers 16Sar and 16Sbr. The data was then analysed using MESQUITE, MEGA, DnaSP and PopART to identify 16S mitochondrial gene polymorphism. This study obtained 610 base pairs after alignment of 16S sequences of all samples. Five haplotypes were detected with 9 variable sites and 2 parsimony informative sites. Haplotype diversity and nucleotide diversity of the spotted barb fish were 1.00 and 0.0066 respectively. In addition, genetic distance among the samples was 0.66. This finding exhibited polymorphism on 16S gene sequence among the fish samples and this data indicated genetic variation of the spotted barb from Lake Lebo Taliwang.
topic barbodes binotatus
16s gene
polymorphism
url https://jurnal.ugm.ac.id/jfs/article/view/54064
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AT nadyaulfanidafirdaus detectionof16smitochondrialgenepolymorphismonbarbfishbarbodesbinotatusvalenciennes1842fromlakelebotaliwangwestnusatenggara
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