MiRNA10b-directed nanotherapy effectively targets brain metastases from breast cancer
Abstract RNA interference represents one of the most appealing therapeutic modalities for cancer because of its potency, versatility, and modularity. Because the mechanism is catalytic and affects the expression of disease-causing antigens at the post-transcriptional level, only small amounts of the...
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doaj-aba3c435f7b44d53a08fc458de111e512021-02-07T12:33:21ZengNature Publishing GroupScientific Reports2045-23222021-02-011111710.1038/s41598-021-82528-2MiRNA10b-directed nanotherapy effectively targets brain metastases from breast cancerByunghee Yoo0Alana Ross1Pamela Pantazopoulos2Zdravka Medarova3MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical SchoolMGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical SchoolMGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical SchoolMGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical SchoolAbstract RNA interference represents one of the most appealing therapeutic modalities for cancer because of its potency, versatility, and modularity. Because the mechanism is catalytic and affects the expression of disease-causing antigens at the post-transcriptional level, only small amounts of therapeutic need to be delivered to the target in order to exert a robust therapeutic effect. RNA interference is also advantageous over other treatment modalities, such as monoclonal antibodies or small molecules, because it has a much broader array of druggable targets. Finally, the complementarity of the genetic code gives us the opportunity to design RNAi therapeutics using computational, rational approaches. Previously, we developed and tested an RNAi-targeted therapeutic, termed MN-anti-miR10b, which was designed to inhibit the critical driver of metastasis and metastatic colonization, miRNA-10b. We showed in animal models of metastatic breast cancer that MN-anti-miR10b accumulated into tumors and metastases in the lymph nodes, lungs, and bone, following simple intravenous injection. We also found that treatment incorporating MN-anti-miR10b was effective at inhibiting the emergence of metastases and could regress already established metastases in the lymph nodes, lungs, and bone. In the present study, we extend the application of MN-anti-miR10b to a model of breast cancer metastatic to the brain. We demonstrate delivery to the metastatic lesions and obtain evidence of a therapeutic effect manifested as inhibition of metastatic progression. This investigation represents an additional step towards translating similar RNAi-targeted therapeutics for the systemic treatment of metastatic disease.https://doi.org/10.1038/s41598-021-82528-2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Byunghee Yoo Alana Ross Pamela Pantazopoulos Zdravka Medarova |
spellingShingle |
Byunghee Yoo Alana Ross Pamela Pantazopoulos Zdravka Medarova MiRNA10b-directed nanotherapy effectively targets brain metastases from breast cancer Scientific Reports |
author_facet |
Byunghee Yoo Alana Ross Pamela Pantazopoulos Zdravka Medarova |
author_sort |
Byunghee Yoo |
title |
MiRNA10b-directed nanotherapy effectively targets brain metastases from breast cancer |
title_short |
MiRNA10b-directed nanotherapy effectively targets brain metastases from breast cancer |
title_full |
MiRNA10b-directed nanotherapy effectively targets brain metastases from breast cancer |
title_fullStr |
MiRNA10b-directed nanotherapy effectively targets brain metastases from breast cancer |
title_full_unstemmed |
MiRNA10b-directed nanotherapy effectively targets brain metastases from breast cancer |
title_sort |
mirna10b-directed nanotherapy effectively targets brain metastases from breast cancer |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-02-01 |
description |
Abstract RNA interference represents one of the most appealing therapeutic modalities for cancer because of its potency, versatility, and modularity. Because the mechanism is catalytic and affects the expression of disease-causing antigens at the post-transcriptional level, only small amounts of therapeutic need to be delivered to the target in order to exert a robust therapeutic effect. RNA interference is also advantageous over other treatment modalities, such as monoclonal antibodies or small molecules, because it has a much broader array of druggable targets. Finally, the complementarity of the genetic code gives us the opportunity to design RNAi therapeutics using computational, rational approaches. Previously, we developed and tested an RNAi-targeted therapeutic, termed MN-anti-miR10b, which was designed to inhibit the critical driver of metastasis and metastatic colonization, miRNA-10b. We showed in animal models of metastatic breast cancer that MN-anti-miR10b accumulated into tumors and metastases in the lymph nodes, lungs, and bone, following simple intravenous injection. We also found that treatment incorporating MN-anti-miR10b was effective at inhibiting the emergence of metastases and could regress already established metastases in the lymph nodes, lungs, and bone. In the present study, we extend the application of MN-anti-miR10b to a model of breast cancer metastatic to the brain. We demonstrate delivery to the metastatic lesions and obtain evidence of a therapeutic effect manifested as inhibition of metastatic progression. This investigation represents an additional step towards translating similar RNAi-targeted therapeutics for the systemic treatment of metastatic disease. |
url |
https://doi.org/10.1038/s41598-021-82528-2 |
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