| Summary: | The sensing and actuation capabilities of biological cells integrated with artificial components have been used to create autonomous microsystems. For creating autonomous microsystems, the unidirectional transport of a submillimeter-sized cargo with stimuli responsive bio-motors should be developed as a fundamental motion. This study aims to use <i>Volvox</i> as a light-controlled microrobot to achieve the unidirectional transport of a submillimeter-sized cargo. We show the fabrication of a guide structure, cargo, and light irradiation platform for a unidirectional actuation. The fundamental performances of each component were investigated, and the motions of <i>Volvox</i> were controlled in a microchamber with the developed light irradiation platform. All components were integrated to demonstrate the unidirectional actuation of a block by <i>Volvox</i>. We discuss the dynamics of the mechanical motions.
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