Selection and validation of endogenous reference genes for qRT-PCR analysis in leafy spurge (Euphorbia esula).
Quantitative real-time polymerase chain reaction (qRT-PCR) is the most important tool in measuring levels of gene expression due to its accuracy, specificity, and sensitivity. However, the accuracy of qRT-PCR analysis strongly depends on transcript normalization using stably expressed reference gene...
Main Authors: | Wun S Chao, Münevver Doğramaci, Michael E Foley, David P Horvath, James V Anderson |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3419244?pdf=render |
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