Molecular fingerprinting of the Indian medicinal plant Strychnos minor Dennst

Molecular fingerprinting of the Indian medicinal plant Strychnos minor Dennst

Genomic DNA of Strychnos minor (Dennst) of sixteen geographical regions were amplified using thirteen primers. The analyses of products of Radom Amplified Polymorphic DNA (RAPD) revealed polymorphism among the samples. The polymorphic information content (PIC) was maximum for the primer of OPB-04 (0...

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Main Authors: T. Arumugam, G. Jayapriya, T. Sekar
Format: Article
Language:English
Published: Elsevier 2019-03-01
Series:Biotechnology Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X19300153
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spelling doaj-ab195aea5c944c1b8163f33803fe2a7a2020-11-25T01:06:37ZengElsevierBiotechnology Reports2215-017X2019-03-0121Molecular fingerprinting of the Indian medicinal plant Strychnos minor DennstT. Arumugam0G. Jayapriya1T. Sekar2Biotech Research and Development, Bioplus Life Science, Hosur, 635109, Tamil Nadu, India; Department of Chemistry, Government Arts and Science College, Dharmapuri, 636701, Tamil Nadu, India; Corresponding author.Biotech Research and Development, Bioplus Life Science, Hosur, 635109, Tamil Nadu, India; Department of Chemistry, Government Arts and Science College, Dharmapuri, 636701, Tamil Nadu, IndiaDivision of Biodiversity and Biotechnology, Post Graduate and Research Department of Botany, Pachaiyappa’s College, Chennai, 600030, Tamil Nadu, India; Department of Chemistry, Government Arts and Science College, Dharmapuri, 636701, Tamil Nadu, IndiaGenomic DNA of Strychnos minor (Dennst) of sixteen geographical regions were amplified using thirteen primers. The analyses of products of Radom Amplified Polymorphic DNA (RAPD) revealed polymorphism among the samples. The polymorphic information content (PIC) was maximum for the primer of OPB-04 (0.40) followed by the primer OPB-20 (0.39). Clustering analysis by Jaccard’s coefficient index exhibited similarity matrix and distance matrix. The amplified products of the 13 primers collectively showed similarity index ranged from 0.654 to 0.986. The phylogenetic trees were constructed by UPGMA cluster analysis. Significant variations in the amplified genomic DNA suggest genetic variability of population but low inter-population genetic segregation. Keywords: Strychnos minor, Molecular fingerprinting, RAPD, Polymorphic Information Contenthttp://www.sciencedirect.com/science/article/pii/S2215017X19300153
collection DOAJ
language English
format Article
sources DOAJ
author T. Arumugam
G. Jayapriya
T. Sekar
spellingShingle T. Arumugam
G. Jayapriya
T. Sekar
Molecular fingerprinting of the Indian medicinal plant Strychnos minor Dennst
Biotechnology Reports
author_facet T. Arumugam
G. Jayapriya
T. Sekar
author_sort T. Arumugam
title Molecular fingerprinting of the Indian medicinal plant Strychnos minor Dennst
title_short Molecular fingerprinting of the Indian medicinal plant Strychnos minor Dennst
title_full Molecular fingerprinting of the Indian medicinal plant Strychnos minor Dennst
title_fullStr Molecular fingerprinting of the Indian medicinal plant Strychnos minor Dennst
title_full_unstemmed Molecular fingerprinting of the Indian medicinal plant Strychnos minor Dennst
title_sort molecular fingerprinting of the indian medicinal plant strychnos minor dennst
publisher Elsevier
series Biotechnology Reports
issn 2215-017X
publishDate 2019-03-01
description Genomic DNA of Strychnos minor (Dennst) of sixteen geographical regions were amplified using thirteen primers. The analyses of products of Radom Amplified Polymorphic DNA (RAPD) revealed polymorphism among the samples. The polymorphic information content (PIC) was maximum for the primer of OPB-04 (0.40) followed by the primer OPB-20 (0.39). Clustering analysis by Jaccard’s coefficient index exhibited similarity matrix and distance matrix. The amplified products of the 13 primers collectively showed similarity index ranged from 0.654 to 0.986. The phylogenetic trees were constructed by UPGMA cluster analysis. Significant variations in the amplified genomic DNA suggest genetic variability of population but low inter-population genetic segregation. Keywords: Strychnos minor, Molecular fingerprinting, RAPD, Polymorphic Information Content
url http://www.sciencedirect.com/science/article/pii/S2215017X19300153
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