Interlaboratory comparison for determination of ochratoxin A by ELISA in maize (running title: Determination of ochratoxin A in maize)

• Main Authors: Jakšić Sandra M., Jajić Igor M., Nešić Ksenija D., Stojanov Igor M., Živkov-Baloš Milica M., Mihaljev Željko A., Abramović Biljana F.
• Format: Article
• Language: English
• Published: Matica srpska 2013-01-01
• Series: Zbornik Matice Srpske za Prirodne Nauke
• Subjects: confirmation methods; ELISA; interlaboratory comparison; maize; ochratoxin A
• Online Access: http://www.doiserbia.nb.rs/img/doi/0352-4906/2013/0352-49061324077J.pdf

Participation in interlaboratory comparison and proficiency testing schemes is important for laboratories to control the work quality. In this study, a sample of naturally contaminated maize was analyzed for the content of ochratoxin A (OTA) in three laboratories in Serbia. Participating laboratories used enzymatic immunoaffinity method (ELISA) for the determination of OTA and selection of the ELISA kit was free. Between-laboratory precision was acceptable as evidenced by Cochran’s C test. Moreover, z-scores for all three laboratories were z < ± 2, which is considered acceptable. Used OTA confirmation methods were thin-layer chromatography (TLC) and high-pressure liquid chromatography (HPLC), with fluorescence detector. The results of different methods were comparable. [Projekat Ministarstva nauke Republike Srbije, br. TR 031071 i br. OI 172042]