New R-Based Methodology to Optimize the Identification of Root Endophytes against <i>Heterobasidion parviporum</i>

New R-Based Methodology to Optimize the Identification of Root Endophytes against <i>Heterobasidion parviporum</i>

Many root fungal endophytes inhabiting forest trees have potential impact on the health and disease progression of certain tree species. Hence, the screening of root endophytes for their biocontrol abilities is relevant for their potential to protect their hosts against invaders. The aim of this res...

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Main Authors: Linda Rigerte, Kathrin Blumenstein, Eeva Terhonen
Format: Article
Language:English
Published: MDPI AG 2019-04-01
Series:Microorganisms
Subjects:
fungal endophytes
inhibitory activity
biocontrol
forest pathogens
conifers
Online Access:https://www.mdpi.com/2076-2607/7/4/102
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spelling doaj-aaf22f3cc9c6442abc2c6678e41ad4102020-11-24T21:20:56ZengMDPI AGMicroorganisms2076-26072019-04-017410210.3390/microorganisms7040102microorganisms7040102New R-Based Methodology to Optimize the Identification of Root Endophytes against <i>Heterobasidion parviporum</i>Linda Rigerte0Kathrin Blumenstein1Eeva Terhonen2Forest Pathology Research Group, Büsgen-Institute, Department of Forest Botany and Tree Physiology, Faculty of Forest Sciences and Forest Ecology, University of Göttingen, Büsgenweg 2, 37077 Göttingen, GermanyForest Pathology Research Group, Büsgen-Institute, Department of Forest Botany and Tree Physiology, Faculty of Forest Sciences and Forest Ecology, University of Göttingen, Büsgenweg 2, 37077 Göttingen, GermanyForest Pathology Research Group, Büsgen-Institute, Department of Forest Botany and Tree Physiology, Faculty of Forest Sciences and Forest Ecology, University of Göttingen, Büsgenweg 2, 37077 Göttingen, GermanyMany root fungal endophytes inhabiting forest trees have potential impact on the health and disease progression of certain tree species. Hence, the screening of root endophytes for their biocontrol abilities is relevant for their potential to protect their hosts against invaders. The aim of this research is to screen for the potential inhibitory effects of selected conifer root endophytes during interaction, in vitro, with the root rot pathogen, <i>Heterobasidion parviporum</i>. Here, we introduce a guideline that facilitates the use of root fungal endophytes as biocontrol agents. We isolated fungal root endophytes from eight different conifers. These root fungal endophytes were evaluated for their antagonism against the root rot pathogen, <i>H</i>. <i>parviporum</i>, by means of paired-culture antagonism assays. We determined the antagonism of the isolated root fungal endophytes to elucidate potential biocontrol applications. For the analysis, a software package in R was developed. Endophyte candidates with antagonistic potential were identified.https://www.mdpi.com/2076-2607/7/4/102fungal endophytesinhibitory activitybiocontrolforest pathogensconifers
collection DOAJ
language English
format Article
sources DOAJ
author Linda Rigerte
Kathrin Blumenstein
Eeva Terhonen
spellingShingle Linda Rigerte
Kathrin Blumenstein
Eeva Terhonen
New R-Based Methodology to Optimize the Identification of Root Endophytes against <i>Heterobasidion parviporum</i>
Microorganisms
fungal endophytes
inhibitory activity
biocontrol
forest pathogens
conifers
author_facet Linda Rigerte
Kathrin Blumenstein
Eeva Terhonen
author_sort Linda Rigerte
title New R-Based Methodology to Optimize the Identification of Root Endophytes against <i>Heterobasidion parviporum</i>
title_short New R-Based Methodology to Optimize the Identification of Root Endophytes against <i>Heterobasidion parviporum</i>
title_full New R-Based Methodology to Optimize the Identification of Root Endophytes against <i>Heterobasidion parviporum</i>
title_fullStr New R-Based Methodology to Optimize the Identification of Root Endophytes against <i>Heterobasidion parviporum</i>
title_full_unstemmed New R-Based Methodology to Optimize the Identification of Root Endophytes against <i>Heterobasidion parviporum</i>
title_sort new r-based methodology to optimize the identification of root endophytes against <i>heterobasidion parviporum</i>
publisher MDPI AG
series Microorganisms
issn 2076-2607
publishDate 2019-04-01
description Many root fungal endophytes inhabiting forest trees have potential impact on the health and disease progression of certain tree species. Hence, the screening of root endophytes for their biocontrol abilities is relevant for their potential to protect their hosts against invaders. The aim of this research is to screen for the potential inhibitory effects of selected conifer root endophytes during interaction, in vitro, with the root rot pathogen, <i>Heterobasidion parviporum</i>. Here, we introduce a guideline that facilitates the use of root fungal endophytes as biocontrol agents. We isolated fungal root endophytes from eight different conifers. These root fungal endophytes were evaluated for their antagonism against the root rot pathogen, <i>H</i>. <i>parviporum</i>, by means of paired-culture antagonism assays. We determined the antagonism of the isolated root fungal endophytes to elucidate potential biocontrol applications. For the analysis, a software package in R was developed. Endophyte candidates with antagonistic potential were identified.
topic fungal endophytes
inhibitory activity
biocontrol
forest pathogens
conifers
url https://www.mdpi.com/2076-2607/7/4/102
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AT kathrinblumenstein newrbasedmethodologytooptimizetheidentificationofrootendophytesagainstiheterobasidionparviporumi
AT eevaterhonen newrbasedmethodologytooptimizetheidentificationofrootendophytesagainstiheterobasidionparviporumi
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