Sulfur is limiting the glucosinolate accumulation in nasturtium <em>in vitro</em> plants (<em>Tropaeolum majus</em> L.)

• Main Authors: L. Matallana, M. Kleinwächter, D. Selmar
• Format: Article
• Language: English
• Published: Julius Kühn-Institut 2012-12-01
• Series: Journal of Applied Botany and Food Quality
• Online Access: https://ojs.openagrar.de/index.php/JABFQ/article/view/2182

It is well established that sulfate fertilisation significantly enhances the content of mustard oil glucosides in glucosinolate containing plants. However, with respect to tissue cultures and in vitro-plants, corresponding data are missing. In this study the influence of sulfur on the accumulation of glucosinolates was analyzed in nasturtium in vitro-plants (Tropaeolum majus). The glucotropaeolin content in plants grown on standard media (MS) varied between 10 and 50 μmol/g DW, corresponding to only about 20 % to 70 % of the glucotropaeolin content in earth grown plants. A fivefold enhancement of the sulfate concentration resulted in a massive increase in the glucotropaeolin content of the in vitro-plants. A decline of sulfate in the medium leads to corresponding diminutions of the glucosinolates accumulated. These data clearly demonstrate the high impact of sulfur availability on glucosinolate biosynthesis and accumulation.