<i>Lactobacillus plantarum</i> CQPC11 Isolated from Sichuan Pickled Cabbages Antagonizes <span style="font-variant: small-caps">d</span>-galactose-Induced Oxidation and Aging in Mice

• Main Authors: Yu Qian, Jing Zhang, Xianrong Zhou, Ruokun Yi, Jianfei Mu, Xingyao Long, Yanni Pan, Xin Zhao, Weiwei Liu
• Format: Article
• Language: English
• Published: MDPI AG 2018-11-01
• Series: Molecules
• Subjects: <i>Lactobacillus plantarum</i> CQPC11; pickled cabbage; oxidation; aging; mice
• Online Access: https://www.mdpi.com/1420-3049/23/11/3026

Chinese pickled cabbage is a traditional fermented food that contains abundant microbes produced during the process of fermentation. In this work, an in vivo animal study was conducted to investigate the effects of a newly isolated lactic acid bacterium (<i>Lactobacillus plantarum</i> CQPC11, LP-CQPC11) on <span style="font-variant: small-caps;">d</span>-galactose-induced oxidation and aging in mice. Analysis of the serum and tissue samples of these mice using molecular biology approaches showed that LP-CQPC11 suppressed the decrease in thymus, brain, heart, liver, spleen, and kidney indices caused by oxidation and aging. Furthermore, LP-CQPC11 increased the levels of SOD (superoxide dismutase), GSH-Px (glutathione peroxidase), and GSH (glutathione), whereas it reduced the levels of NO (nitric oxide) and MDA (malondialdehyde) in the serum, liver, and spleen of oxidation and aging mouse models. Pathological observation indicated that LP-CQPC11 alleviated the damage caused by oxidation and aging on the liver and spleen of mice. qPCR analysis indicated that LP-CQPC11 effectively upregulated the expression of <i>nNOS</i> (neuronal nitric oxide synthase), <i>eNOS</i> (endothelial nitric oxide synthase), <i>Cu/Zn-SOD</i> (cuprozinc-superoxide dismutase), <i>Mn-SOD</i> (manganese superoxide dismutase), <i>CAT</i> (catalase), <i>HO-1</i> (heme oxygenase-1), <i>Nrf2</i> (nuclear factor-erythroid 2 related factor 2), &#947;<i>-GCS</i> (&#947;-glutamylcysteine synthetase), and <i>NQO1</i> (NAD(P)H dehydrogenase [quinone] 1), but downregulated the expression of <i>iNOS</i> (inducible nitric oxide synthase) in the mouse liver and spleen. Western blot analysis showed that LP-CQPC11 effectively upregulated SOD1 (<i>Cu/Zn-SOD</i>), SOD2 (<i>Mn-SOD</i>), CAT, GSH1 (c-glutamylcysteine synthetase), and GSH2 (glutathione synthetase) protein expression in mouse liver and spleen tissues. These findings suggest that LP-CQPC11 can effectively prevent <span style="font-variant: small-caps;">d</span>-galactose-induced oxidation and aging in mice, and the effect is even better than that of the commonly used <i>Lactobacillus delbruechii</i> subsp. <i>bulgaricus</i> (LDSB) and vitamin C in the industry. Thus, LP-CQPC11 may be potentially employed as a probiotic strain.