The study of expression of PTEN and AKT1 genes in co-culturing of HT29 colon cancer cell line with Streptococcus thermophilus

• Main Authors: Shiva Sattari, Changiz Ahmadizadeh
• Format: Article
• Language: fas
• Published: Kashan University of Medical Sciences and Health Services 2019-02-01
• Series: Fiyz̤
• Subjects: Probiotic; Colon cancer; HT29 cell
• Online Access: http://feyz.kaums.ac.ir/article-1-3648-en.html
• ISSN: 1029-7855; 2008-9821

Background: Colon cancer is one of the most common cancers in the world. Probiotics are viable and useful microorganisms that have an effective role in controlling cancer by influencing the digestive enzymes of animals and humans, inhibition of cancerous agents in the body and in vitro conditions, suppression of lotions, and cancer-inducing compounds and tumors in experimental animals. The present study was conducted to investigate the PTEN/ AKT1 cellular signaling pathway in adjacent cultures of Streptococcus thermophilus with HT29 colon cancer cells. Materials and Methods: Bacterial culture, supernatant and bacterial extract were prepared and the cells were treated with these materials. Cell necrosis was evaluated by the MTT method. Also, the expression of PTEN and AKT1 genes in HT29 cell line was investigated using the real-time PCR. Results: The results showed that the thermophiles bacterium reduced the expression of AKT1 genes, and increased the expression of PTEN and led the cancerous cells toward apoptosis. The MTT test showed that the concentration of OD=0.05 had the highest mortality in 4 hours. Conclusion: Thermophiles bacteria can be used to create a novel therapeutic effect with high impact, low side effects, harmless biological effect and lower costs. Also, it can be used as an extra treatment appropriate to the body's natural flora for the treatment and prevention of colon cancer.