Indication and identification of infectious agents in animal products based on biochip technology with a biotin label and colorimetric detection

• Main Authors: A V Lokorev, I M Nityaga, D V Fedyushin, N G Khomenets, L A Shamanova
• Format: Article
• Language: English
• Published: Peoples’ Friendship University of Russia (RUDN University) 2016-12-01
• Series: RUDN Journal of Agronomy and Animal Industries
• Subjects: чип; биочип; микрочиповая технология; биотиновая метка; идентификация возбудителей инфекций
• Online Access: http://journals.rudn.ru/agronomy/article/view/13953

The results of the development of DNA chips with biotin uterus and colorimetric detection to indicate and identify pathogens in animal products. The constant need to monitor for dangerous infectious diseases makes the urgency of developing new effective methods of identification of pathogens of these diseases. The high specificity and sensitivity of the technique, is 103-104 bacterial cells. The analysis takes 5-7 hours. Visual assessment of the end result allows for biochip test systems in stationary and field conditions, laboratories and regional reference centers. The application of modern biochip technology research quickly and with high reliability to detect infectious agents in a variety of biological materials, typified by strains in order to establish a common source of infection.