Mechanism of Action of <i>Mangifera indica</i> Leaves for Anti-Diabetic Activity

• Main Authors: Dai-Hung Ngo, Dai-Nghiep Ngo, Thi Thanh Nhan Vo, Thanh Sang Vo
• Format: Article
• Language: English
• Published: Österreichische Apotheker-Verlagsgesellschaft m. b. H. 2019-05-01
• Series: Scientia Pharmaceutica
• Subjects: herbal plant; alpha-amylase; anti-diabetes; NO production; glucose uptake
• Online Access: https://www.mdpi.com/2218-0532/87/2/13

Diabetes is a major metabolic disorder whose prevalence is increasing daily. Medicinal plants have played an important role in the prevention and treatment of type 2 diabetes via prophylactic and therapeutic management. In this study, <i>Mangifera Indica</i> leaf (MIL) extract was investigated for its promising anti-diabetic activity via an in vitro model. It was found that MIL extract possessed significant inhibition on alpha-amylase activity up to (51.4 &#177; 2.7)% at a concentration of 200 &#181;g/mL. Moreover, glucose adsorption capacity of MIL was identified at (2.7 &#177; 0.19) mM glucose/g extract. Furthermore, the extract caused a significant increase in glucose uptake up to (143 &#177; 9.3)% in LO-2 liver cells. Notably, MIL extract was effective in scavenging (63.3 &#177; 2.1)% 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and (71.6 &#177; 4.3)% 2,2-azinobis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS)⁺ radicals and inhibiting (66 &#177; 4.9)% NO production from RAW264.7 cells without any cytotoxicity effects. Accordingly, <i>M. indica</i> leaves are suggested as a promising material for development of hypoglycemic products.