Application of the ATP Bioluminescence Method in Rapid Detection of Microorganisms in Jet Fuel

• Main Authors: Xinfeng Sun, Yun Xiong, Peng Su, Peng Zhu, Mingming Niu, Dongliang Zhou
• Format: Article
• Language: English
• Published: Bulgarian Academy of Sciences 2019-09-01
• Series: International Journal Bioautomation
• Subjects: atp bioluminescence method; jet fuel; contamination fungi; detection
• Online Access: http://www.biomed.bas.bg/bioautomation/2019/vol_23.3/files/23.3_07.pdf

Rapid detection of microorganisms in jet fuel can effectively prevent a series of problems caused by microbial contamination. In this paper, the application of the ATP bioluminescence method developed by the research group is systematically studied in the rapid detection of microorganisms in jet fuel. The results show that the ATP standard curve of the ATP bioluminescence method has good linear relationships with the standard curves of the colonies of five major contamination fungi and mixed bacteria (R2 is all above 0.96), and that the ATP content in each single colony is on the order of magnitude of 10-17 mol, indicating that this method can achieve rapid and effective measurement of the total number of microorganisms in jet fuel. The method also proves t have good measuring performance in various aspects – the method detection limit is 8.8x10-17 mol and the repeatability and reproducibility is 29.35%, which are within the acceptable range; the fluorescence reaction is highly stable, and a fluorescence decay of 5% can be guaranteed within 60s. This method has little difference in field detection with the HY-LITE® JET A1 fuel test method recommended by IATA, and what is more, it is accurate, stable and lost-cost, making it effective as an alternative method. It is an independent intellectual property of China and can be recommended as a method for rapid detection of microorganisms in jet fuel in national standards.