Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase

Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase

Reveromycin A (RM-A) selectively inhibits eukaryotic cytoplasmic isoleucyltRNA synthetase (IleRS). Herein, the authors show that RM-A molecule occupies the tRNAIle binding site of Saccharomyces cerevisiae IleRS, and that RM-A cooperates with isoleucine or isoleucyl-adenylate for IleRS binding.

Bibliographic Details
Main Authors: Bingyi Chen, Siting Luo, Songxuan Zhang, Yingchen Ju, Qiong Gu, Jun Xu, Xiang-Lei Yang, Huihao Zhou
Format: Article
Language:English
Published: Nature Publishing Group 2021-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-21902-0
id doaj-aa106fd7577240a4b8e1ff9e7d96b4fa
record_format Article
spelling doaj-aa106fd7577240a4b8e1ff9e7d96b4fa2021-03-14T12:07:01ZengNature Publishing GroupNature Communications2041-17232021-03-0112111010.1038/s41467-021-21902-0Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetaseBingyi Chen0Siting Luo1Songxuan Zhang2Yingchen Ju3Qiong Gu4Jun Xu5Xiang-Lei Yang6Huihao Zhou7Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, Sun Yat-sen UniversityResearch Center for Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-sen UniversityResearch Center for Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-sen UniversityDepartment of Molecular Medicine, Scripps Research InstituteGuangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, Sun Yat-sen UniversityReveromycin A (RM-A) selectively inhibits eukaryotic cytoplasmic isoleucyltRNA synthetase (IleRS). Herein, the authors show that RM-A molecule occupies the tRNAIle binding site of Saccharomyces cerevisiae IleRS, and that RM-A cooperates with isoleucine or isoleucyl-adenylate for IleRS binding.https://doi.org/10.1038/s41467-021-21902-0
collection DOAJ
language English
format Article
sources DOAJ
author Bingyi Chen
Siting Luo
Songxuan Zhang
Yingchen Ju
Qiong Gu
Jun Xu
Xiang-Lei Yang
Huihao Zhou
spellingShingle Bingyi Chen
Siting Luo
Songxuan Zhang
Yingchen Ju
Qiong Gu
Jun Xu
Xiang-Lei Yang
Huihao Zhou
Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase
Nature Communications
author_facet Bingyi Chen
Siting Luo
Songxuan Zhang
Yingchen Ju
Qiong Gu
Jun Xu
Xiang-Lei Yang
Huihao Zhou
author_sort Bingyi Chen
title Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase
title_short Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase
title_full Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase
title_fullStr Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase
title_full_unstemmed Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase
title_sort inhibitory mechanism of reveromycin a at the trna binding site of a class i synthetase
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2021-03-01
description Reveromycin A (RM-A) selectively inhibits eukaryotic cytoplasmic isoleucyltRNA synthetase (IleRS). Herein, the authors show that RM-A molecule occupies the tRNAIle binding site of Saccharomyces cerevisiae IleRS, and that RM-A cooperates with isoleucine or isoleucyl-adenylate for IleRS binding.
url https://doi.org/10.1038/s41467-021-21902-0
work_keys_str_mv AT bingyichen inhibitorymechanismofreveromycinaatthetrnabindingsiteofaclassisynthetase
AT sitingluo inhibitorymechanismofreveromycinaatthetrnabindingsiteofaclassisynthetase
AT songxuanzhang inhibitorymechanismofreveromycinaatthetrnabindingsiteofaclassisynthetase
AT yingchenju inhibitorymechanismofreveromycinaatthetrnabindingsiteofaclassisynthetase
AT qionggu inhibitorymechanismofreveromycinaatthetrnabindingsiteofaclassisynthetase
AT junxu inhibitorymechanismofreveromycinaatthetrnabindingsiteofaclassisynthetase
AT xiangleiyang inhibitorymechanismofreveromycinaatthetrnabindingsiteofaclassisynthetase
AT huihaozhou inhibitorymechanismofreveromycinaatthetrnabindingsiteofaclassisynthetase
_version_ 1724221585330536448

Similar Items