Use of nested PCR for the detection of trichomonads in bronchoalveolar lavage fluid

Use of nested PCR for the detection of trichomonads in bronchoalveolar lavage fluid

Abstract Background The methods routinely used to detect trichomonads in the lungs are not sensitive enough, and an effective method is urgently needed. Method Primers were first designed to match the conserved area of the 18S rRNA gene of trichomonads. Then, nested PCR was carried out to detect tri...

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Bibliographic Details
Main Authors: Chao Lin, Furong Ying, Yanan Lai, Xiaolong Li, Xiangyang Xue, Tieli Zhou, Dongwei Hu
Format: Article
Language:English
Published: BMC 2019-06-01
Series:BMC Infectious Diseases
Subjects:
Bronchoalveolar lavage fluid
Nested PCR
Trichomonad
Lung
Online Access:http://link.springer.com/article/10.1186/s12879-019-4118-9

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