Potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats

• Main Authors: Muhammed Yayla, Damla Cetin, Yasemen Adali, Pinar Aksu Kilicle, Erdem Toktay
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2018-12-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: Ischemia/Reperfusion; Oxidative stress; Ovary; Pomegranate seed oil; Punicaceae; Rats
• Online Access: http://ijbms.mums.ac.ir/article_11675_7fecec992dbedc728ecc78c8f605e28d.pdf

Objective(s): The aim of this study is to determine the therapeutic effects of pomegranate seed oil, which is a powerful antioxidant and anti-inflammatory agent, on ovarian-ischemia and reperfusion injury in rats.Materials and Methods: Fifty-six  female albino Wistar rats were divided into 7 equal groups. Group 1; Sham Operation, Group 2; Ischemia, Group 3; Ischemia + Reperfusion, Group 4; Ischemia + Pomegranate 0,32 ml / kg (IP), Group 5; Ischemia + Pomegranate 0.64 ml / kg, Group 6; Ischemia + Pomegranate 0,32 ml / kg + reperfusion, Group 7; Ischemia + Pomegranate 0,64 ml / kg + reperfusion. Three hours after ischemia and 3 hours after reperfusion, the study was terminated. Results: While NADPH oxidase activity, MDA and TNF-α levels were significantly increased, SOD activity and GSH levels were reduced in ischemia and I/R groups. Low dose pomegranate seed oil  application reduced significantly oxidative stress and NADPH oxidase activity in both ischemic and ischemic/reperfusion groups. At the same time, low-dose pomegranate seed oil extract reduced TNF-α levels and significantly increased antioxidant activity.Conclusion: PSO demonstrated an important therapeutic effect in the treatment of ovarian ischemia and reperfusion injury.