c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S]

Oxidative stress, hepatic steatosis, and mitochondrial dysfunction are key pathophysiological features of nonalcoholic fatty liver disease. A conjugated linoleic acid (CLA) mixture of cis9,trans11 (9,11-CLA) and trans10,cis12 (10,12-CLA) isomers enhanced the antioxidant/detoxifying mechanism via the...

Full description

Bibliographic Details
Main Authors: Maria Pina Mollica, Giovanna Trinchese, Gina Cavaliere, Chiara De Filippo, Ennio Cocca, Marcello Gaita, Antonio Della-Gatta, Angela Marano, Giuseppe Mazzarella, Paolo Bergamo
Format: Article
Language:English
Published: Elsevier 2014-05-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520375039
id doaj-a9afb54e11ee4fc4981ce7d57f794016
record_format Article
spelling doaj-a9afb54e11ee4fc4981ce7d57f7940162021-04-28T06:02:03ZengElsevierJournal of Lipid Research0022-22752014-05-01555837849c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S]Maria Pina Mollica0Giovanna Trinchese1Gina Cavaliere2Chiara De Filippo3Ennio Cocca4Marcello Gaita5Antonio Della-Gatta6Angela Marano7Giuseppe Mazzarella8Paolo Bergamo9Dipartimento delle Scienze Biologiche, Università degli Studi di Napoli “Federico II,” Napoli, ItalyDipartimento delle Scienze Biologiche, Università degli Studi di Napoli “Federico II,” Napoli, ItalyDipartimento delle Scienze Biologiche, Università degli Studi di Napoli “Federico II,” Napoli, ItalyDipartimento delle Scienze Biologiche, Università degli Studi di Napoli “Federico II,” Napoli, ItalyInstitute of Biosciences and BioResources, National Research Council (CNR-IBBR), Napoli, ItalyDipartimento delle Scienze Biologiche, Università degli Studi di Napoli “Federico II,” Napoli, ItalyDipartimento delle Scienze Biologiche, Università degli Studi di Napoli “Federico II,” Napoli, ItalyInstitute of Food Sciences, National Research Council (CNR-ISA), Avellino, ItalyInstitute of Food Sciences, National Research Council (CNR-ISA), Avellino, ItalyTo whom correspondence should be addressed; Institute of Food Sciences, National Research Council (CNR-ISA), Avellino, ItalyOxidative stress, hepatic steatosis, and mitochondrial dysfunction are key pathophysiological features of nonalcoholic fatty liver disease. A conjugated linoleic acid (CLA) mixture of cis9,trans11 (9,11-CLA) and trans10,cis12 (10,12-CLA) isomers enhanced the antioxidant/detoxifying mechanism via the activation of nuclear factor E2-related factor-2 (Nrf2) and improved mitochondrial function, but less is known about the actions of specific isomers. The differential ability of individual CLA isomers to modulate these pathways was explored in Wistar rats fed for 4 weeks with a lard-based high-fat diet (L) or with control diet (CD), and, within each dietary treatment, two subgroups were daily administered with 9,11-CLA or 10,12-CLA (30 mg/day). The 9,11-CLA, but not 10,12-CLA, supplementation to CD rats improves the GSH/GSSG ratio in the liver, mitochondrial functions, and Nrf2 activity. Histological examination reveals a reduction of steatosis in L-fed rats supplemented with both CLA isomers, but 9,11-CLA downregulated plasma concentrations of proinflammatory markers, mitochondrial dysfunction, and oxidative stress markers in liver more efficiently than in 10,12-CLA treatment. The present study demonstrates the higher protective effect of 9,11-CLA against diet-induced pro-oxidant and proinflammatory signs and suggests that these effects are determined, at least in part, by its ability to activate the Nrf2 pathway and to improve the mitochondrial functioning and biogenesis.http://www.sciencedirect.com/science/article/pii/S0022227520375039nuclear factor E2-related factor-2fatty acidsmitochondrial efficiency
collection DOAJ
language English
format Article
sources DOAJ
author Maria Pina Mollica
Giovanna Trinchese
Gina Cavaliere
Chiara De Filippo
Ennio Cocca
Marcello Gaita
Antonio Della-Gatta
Angela Marano
Giuseppe Mazzarella
Paolo Bergamo
spellingShingle Maria Pina Mollica
Giovanna Trinchese
Gina Cavaliere
Chiara De Filippo
Ennio Cocca
Marcello Gaita
Antonio Della-Gatta
Angela Marano
Giuseppe Mazzarella
Paolo Bergamo
c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S]
Journal of Lipid Research
nuclear factor E2-related factor-2
fatty acids
mitochondrial efficiency
author_facet Maria Pina Mollica
Giovanna Trinchese
Gina Cavaliere
Chiara De Filippo
Ennio Cocca
Marcello Gaita
Antonio Della-Gatta
Angela Marano
Giuseppe Mazzarella
Paolo Bergamo
author_sort Maria Pina Mollica
title c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S]
title_short c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S]
title_full c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S]
title_fullStr c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S]
title_full_unstemmed c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S]
title_sort c9,t11-conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and nrf2 pathway[s]
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 2014-05-01
description Oxidative stress, hepatic steatosis, and mitochondrial dysfunction are key pathophysiological features of nonalcoholic fatty liver disease. A conjugated linoleic acid (CLA) mixture of cis9,trans11 (9,11-CLA) and trans10,cis12 (10,12-CLA) isomers enhanced the antioxidant/detoxifying mechanism via the activation of nuclear factor E2-related factor-2 (Nrf2) and improved mitochondrial function, but less is known about the actions of specific isomers. The differential ability of individual CLA isomers to modulate these pathways was explored in Wistar rats fed for 4 weeks with a lard-based high-fat diet (L) or with control diet (CD), and, within each dietary treatment, two subgroups were daily administered with 9,11-CLA or 10,12-CLA (30 mg/day). The 9,11-CLA, but not 10,12-CLA, supplementation to CD rats improves the GSH/GSSG ratio in the liver, mitochondrial functions, and Nrf2 activity. Histological examination reveals a reduction of steatosis in L-fed rats supplemented with both CLA isomers, but 9,11-CLA downregulated plasma concentrations of proinflammatory markers, mitochondrial dysfunction, and oxidative stress markers in liver more efficiently than in 10,12-CLA treatment. The present study demonstrates the higher protective effect of 9,11-CLA against diet-induced pro-oxidant and proinflammatory signs and suggests that these effects are determined, at least in part, by its ability to activate the Nrf2 pathway and to improve the mitochondrial functioning and biogenesis.
topic nuclear factor E2-related factor-2
fatty acids
mitochondrial efficiency
url http://www.sciencedirect.com/science/article/pii/S0022227520375039
work_keys_str_mv AT mariapinamollica c9t11conjugatedlinoleicacidamelioratessteatosisbymodulatingmitochondrialuncouplingandnrf2pathways
AT giovannatrinchese c9t11conjugatedlinoleicacidamelioratessteatosisbymodulatingmitochondrialuncouplingandnrf2pathways
AT ginacavaliere c9t11conjugatedlinoleicacidamelioratessteatosisbymodulatingmitochondrialuncouplingandnrf2pathways
AT chiaradefilippo c9t11conjugatedlinoleicacidamelioratessteatosisbymodulatingmitochondrialuncouplingandnrf2pathways
AT enniococca c9t11conjugatedlinoleicacidamelioratessteatosisbymodulatingmitochondrialuncouplingandnrf2pathways
AT marcellogaita c9t11conjugatedlinoleicacidamelioratessteatosisbymodulatingmitochondrialuncouplingandnrf2pathways
AT antoniodellagatta c9t11conjugatedlinoleicacidamelioratessteatosisbymodulatingmitochondrialuncouplingandnrf2pathways
AT angelamarano c9t11conjugatedlinoleicacidamelioratessteatosisbymodulatingmitochondrialuncouplingandnrf2pathways
AT giuseppemazzarella c9t11conjugatedlinoleicacidamelioratessteatosisbymodulatingmitochondrialuncouplingandnrf2pathways
AT paolobergamo c9t11conjugatedlinoleicacidamelioratessteatosisbymodulatingmitochondrialuncouplingandnrf2pathways
_version_ 1721504507926413312