c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S]
Oxidative stress, hepatic steatosis, and mitochondrial dysfunction are key pathophysiological features of nonalcoholic fatty liver disease. A conjugated linoleic acid (CLA) mixture of cis9,trans11 (9,11-CLA) and trans10,cis12 (10,12-CLA) isomers enhanced the antioxidant/detoxifying mechanism via the...
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doaj-a9afb54e11ee4fc4981ce7d57f7940162021-04-28T06:02:03ZengElsevierJournal of Lipid Research0022-22752014-05-01555837849c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S]Maria Pina Mollica0Giovanna Trinchese1Gina Cavaliere2Chiara De Filippo3Ennio Cocca4Marcello Gaita5Antonio Della-Gatta6Angela Marano7Giuseppe Mazzarella8Paolo Bergamo9Dipartimento delle Scienze Biologiche, Università degli Studi di Napoli “Federico II,” Napoli, ItalyDipartimento delle Scienze Biologiche, Università degli Studi di Napoli “Federico II,” Napoli, ItalyDipartimento delle Scienze Biologiche, Università degli Studi di Napoli “Federico II,” Napoli, ItalyDipartimento delle Scienze Biologiche, Università degli Studi di Napoli “Federico II,” Napoli, ItalyInstitute of Biosciences and BioResources, National Research Council (CNR-IBBR), Napoli, ItalyDipartimento delle Scienze Biologiche, Università degli Studi di Napoli “Federico II,” Napoli, ItalyDipartimento delle Scienze Biologiche, Università degli Studi di Napoli “Federico II,” Napoli, ItalyInstitute of Food Sciences, National Research Council (CNR-ISA), Avellino, ItalyInstitute of Food Sciences, National Research Council (CNR-ISA), Avellino, ItalyTo whom correspondence should be addressed; Institute of Food Sciences, National Research Council (CNR-ISA), Avellino, ItalyOxidative stress, hepatic steatosis, and mitochondrial dysfunction are key pathophysiological features of nonalcoholic fatty liver disease. A conjugated linoleic acid (CLA) mixture of cis9,trans11 (9,11-CLA) and trans10,cis12 (10,12-CLA) isomers enhanced the antioxidant/detoxifying mechanism via the activation of nuclear factor E2-related factor-2 (Nrf2) and improved mitochondrial function, but less is known about the actions of specific isomers. The differential ability of individual CLA isomers to modulate these pathways was explored in Wistar rats fed for 4 weeks with a lard-based high-fat diet (L) or with control diet (CD), and, within each dietary treatment, two subgroups were daily administered with 9,11-CLA or 10,12-CLA (30 mg/day). The 9,11-CLA, but not 10,12-CLA, supplementation to CD rats improves the GSH/GSSG ratio in the liver, mitochondrial functions, and Nrf2 activity. Histological examination reveals a reduction of steatosis in L-fed rats supplemented with both CLA isomers, but 9,11-CLA downregulated plasma concentrations of proinflammatory markers, mitochondrial dysfunction, and oxidative stress markers in liver more efficiently than in 10,12-CLA treatment. The present study demonstrates the higher protective effect of 9,11-CLA against diet-induced pro-oxidant and proinflammatory signs and suggests that these effects are determined, at least in part, by its ability to activate the Nrf2 pathway and to improve the mitochondrial functioning and biogenesis.http://www.sciencedirect.com/science/article/pii/S0022227520375039nuclear factor E2-related factor-2fatty acidsmitochondrial efficiency |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Pina Mollica Giovanna Trinchese Gina Cavaliere Chiara De Filippo Ennio Cocca Marcello Gaita Antonio Della-Gatta Angela Marano Giuseppe Mazzarella Paolo Bergamo |
spellingShingle |
Maria Pina Mollica Giovanna Trinchese Gina Cavaliere Chiara De Filippo Ennio Cocca Marcello Gaita Antonio Della-Gatta Angela Marano Giuseppe Mazzarella Paolo Bergamo c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S] Journal of Lipid Research nuclear factor E2-related factor-2 fatty acids mitochondrial efficiency |
author_facet |
Maria Pina Mollica Giovanna Trinchese Gina Cavaliere Chiara De Filippo Ennio Cocca Marcello Gaita Antonio Della-Gatta Angela Marano Giuseppe Mazzarella Paolo Bergamo |
author_sort |
Maria Pina Mollica |
title |
c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S] |
title_short |
c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S] |
title_full |
c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S] |
title_fullStr |
c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S] |
title_full_unstemmed |
c9,t11-Conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[S] |
title_sort |
c9,t11-conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and nrf2 pathway[s] |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2014-05-01 |
description |
Oxidative stress, hepatic steatosis, and mitochondrial dysfunction are key pathophysiological features of nonalcoholic fatty liver disease. A conjugated linoleic acid (CLA) mixture of cis9,trans11 (9,11-CLA) and trans10,cis12 (10,12-CLA) isomers enhanced the antioxidant/detoxifying mechanism via the activation of nuclear factor E2-related factor-2 (Nrf2) and improved mitochondrial function, but less is known about the actions of specific isomers. The differential ability of individual CLA isomers to modulate these pathways was explored in Wistar rats fed for 4 weeks with a lard-based high-fat diet (L) or with control diet (CD), and, within each dietary treatment, two subgroups were daily administered with 9,11-CLA or 10,12-CLA (30 mg/day). The 9,11-CLA, but not 10,12-CLA, supplementation to CD rats improves the GSH/GSSG ratio in the liver, mitochondrial functions, and Nrf2 activity. Histological examination reveals a reduction of steatosis in L-fed rats supplemented with both CLA isomers, but 9,11-CLA downregulated plasma concentrations of proinflammatory markers, mitochondrial dysfunction, and oxidative stress markers in liver more efficiently than in 10,12-CLA treatment. The present study demonstrates the higher protective effect of 9,11-CLA against diet-induced pro-oxidant and proinflammatory signs and suggests that these effects are determined, at least in part, by its ability to activate the Nrf2 pathway and to improve the mitochondrial functioning and biogenesis. |
topic |
nuclear factor E2-related factor-2 fatty acids mitochondrial efficiency |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520375039 |
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