CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum

Corynebacterium glutamicum is an important industrial microbe, however it has proven difficult to genetically engineer using Cas9 from Streptococcus pyogenes. Here the authors report effective genome engineering of the bacterium using Cpf1 from Francisella novicida.

Bibliographic Details
Main Authors:	Yu Jiang, Fenghui Qian, Junjie Yang, Yingmiao Liu, Feng Dong, Chongmao Xu, Bingbing Sun, Biao Chen, Xiaoshu Xu, Yan Li, Renxiao Wang, Sheng Yang
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-05-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms15179

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spelling	doaj-a9a9e34e503a4764a901ee70b6240d592021-05-11T07:34:15ZengNature Publishing GroupNature Communications2041-17232017-05-018111110.1038/ncomms15179CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicumYu Jiang0Fenghui Qian1Junjie Yang2Yingmiao Liu3Feng Dong4Chongmao Xu5Bingbing Sun6Biao Chen7Xiaoshu Xu8Yan Li9Renxiao Wang10Sheng Yang11Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesKey Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesKey Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesKey Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesKey Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesKey Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesKey Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesKey Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesKey Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesState Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of SciencesState Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of SciencesKey Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesCorynebacterium glutamicum is an important industrial microbe, however it has proven difficult to genetically engineer using Cas9 from Streptococcus pyogenes. Here the authors report effective genome engineering of the bacterium using Cpf1 from Francisella novicida.https://doi.org/10.1038/ncomms15179
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Yu Jiang Fenghui Qian Junjie Yang Yingmiao Liu Feng Dong Chongmao Xu Bingbing Sun Biao Chen Xiaoshu Xu Yan Li Renxiao Wang Sheng Yang
spellingShingle	Yu Jiang Fenghui Qian Junjie Yang Yingmiao Liu Feng Dong Chongmao Xu Bingbing Sun Biao Chen Xiaoshu Xu Yan Li Renxiao Wang Sheng Yang CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum Nature Communications
author_facet	Yu Jiang Fenghui Qian Junjie Yang Yingmiao Liu Feng Dong Chongmao Xu Bingbing Sun Biao Chen Xiaoshu Xu Yan Li Renxiao Wang Sheng Yang
author_sort	Yu Jiang
title	CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum
title_short	CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum
title_full	CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum
title_fullStr	CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum
title_full_unstemmed	CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum
title_sort	crispr-cpf1 assisted genome editing of corynebacterium glutamicum
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2017-05-01
description	Corynebacterium glutamicum is an important industrial microbe, however it has proven difficult to genetically engineer using Cas9 from Streptococcus pyogenes. Here the authors report effective genome engineering of the bacterium using Cpf1 from Francisella novicida.
url	https://doi.org/10.1038/ncomms15179
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CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum

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