Study on the value of ANA, ANCA, HLA-B27 and anti-dsDNA in the diagnosis of immune-related uveitis

• Main Authors: Bing Wang, Xian Liu
• Format: Article
• Language: English
• Published: Press of International Journal of Ophthalmology (IJO PRESS) 2018-01-01
• Series: Guoji Yanke Zazhi
• Subjects: immune-related uveitis; antigen; antibody; diagnosis
• Online Access: http://ies.ijo.cn/cn_publish/2018/1/201801025.pdf
• ISSN: 1672-5123; 1672-5123

AIM: To investigate the value of ANA, ANCA, HLA-B27, anti-dsDNA in the diagnosis of immune-related uveitis. <p>METHODS: Totally 70 patients with immune-related uveitis were selected and the clinical data were collected. The levels of ANA, ANCA, anti-dsDNA in serum and HLA-B27 in peripheral blood lymphocytes were measured.<p>RESULTS: Male patients were younger than femle patients(<i>t</i>=2.845, <i>P</i><0.05); there were 42 cases, 12 cases, 6 cases, 5 cases, 4 cases, 1 case, repectively in spondyloarthritis, primary systemic vasculitis, systemic lupus erythematosus, primary Sjogren's syndrome, rheumatoid arthritis and juvenile idiopathic arthritis among the 70 patients with immune-related uveitis. The proportion of spondylitis and systemic lupus erythematosus between men and women had significant difference(<i>P</i>=0.019, 0.008). The ANA positive rate was 16%(11/70), the positive rate of ANCA was 10%(7/70), the positive rate of HLA-B27 was 59%(41/70), the positive rate of anti-dsDNA was 6%(4/70). Among the cases, in male ANA, ANCA, anti-dsDNA positive rate was lower than in women(<i>P</i><0.05). The positive rate of HLA-B27 in male was higher than that in female, the difference was significant(<i>χ</i>²=4.427, <i>P</i><0.05). The main site of the disease in 70 cases of immune-related uveitis were the anterior uvea(49%)and the entire uvea(46%). ANA was mainly expressed in systemic lupus erythematosus(83%)and primary Sjogren's syndrome(60%); ANCA was expressed in immune-related uveitis(except for spondyloarthritis and juvenile idiopathic arthritis); HLA-B27 was predominantly expressed in spondylarthritis(93%); anti-dsDNA was mainly expressed in systemic lupus erythematosus(67%)and rheumatoid arthritis(25%).<p>CONCLUSION: The positive rates of ANA, ANCA, HLA-B27 and anti-dsDNA are different in different immunological-related uveitis, which has a certain diagnostic effect on the classification of immune-related uveitis.