Detection of submicroscopic infection with plasmodium spp., using classical and molecular techniques in pregnant patient from Córdoba, Colombia = Detección de casos submicroscópicos de Plasmodium spp., utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia

• Main Authors: Blanquiceth, Yurany Patricia, Murillo Gómez, Óscar, Maestre, Amanda Elena, Corredor, Mauricio
• Format: Article
• Language: Spanish
• Published: Universidad de Antioquia 2014-07-01
• Series: Iatreia
• Subjects: Gestational Malaria; Malaria; Plasmodium falciparum; Plasmodium vivax; Thick Blood Smear; Real-Time and Quantitative PCR (qRT-PCR); Embarazo; Gota Gruesa; PCR en tiempo real y cuantitativa (qRT-PCR)
• Online Access: http://aprendeenlinea.udea.edu.co/revistas/index.php/iatreia/article/view/13574/16763
• ISSN: 0121-0793; 2011-7965

Introduction: Gestational malaria affects both the mother and the development of her embryo or fetus. Rapid diagnosis and timely and effective treatment are required to prevent complications and deaths. Objective: To compare thick blood smear with nested PCR and real-time PCR (qRT-PCR) for the diagnosis of submicroscopic infections with Plasmodium falciparum and P. vivax. Methodology: 21 women with clinical manifestations of malaria, including both pregnant and non-pregnant, were studied in Puerto Libertador, Córdoba, Colombia. Peripheral blood specimens were obtained from all of them; umbilical cord and placenta blood specimens were taken in the pregnant ones. DNA was extracted and amplified for nested PCR or qRT-PCR. Statistical analysis was done using Graphpad PRISM and EPIDAT softwares. Results: The three techniques were satisfactory for the detection of Plasmodium falciparum and P. vivax in peripheral blood and in the umbilical cord and placenta specimens. Molecular tests were 100% sensitive and specific. Two submicroscopic cases of P. falciparum infection were detected with the two PCR techniques. Conclusion: qRT-PCR is advantageous over nested PCR because its standardization is shorter, it requires lesser infrastructure and it allows the quantification of DNA.