A diacylglycerol photoswitching protocol for studying TRPC channel functions in mammalian cells and tissue slices

Summary: Small molecular probes designed for photopharmacology and opto-chemogenetics are rapidly gaining widespread recognition for investigations of transient receptor potential canonical (TRPC) channels. This protocol describes the use of three photoswitchable diacylglycerol analogs—PhoDAG-1, Pho...

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Bibliographic Details
Main Authors: Trese Leinders-Zufall, Ursula Storch, Michael Mederos y Schnitzler, Navin K. Ojha, Kohei Koike, Thomas Gudermann, Frank Zufall
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002343