Changes in nucleosome occupancy occur in a chromosome specific manner

• Main Authors: Brittany S. Sexton, Brooke R. Druliner, Denis Avey, Fanxiu Zhu, Jonathan H. Dennis
• Format: Article
• Language: English
• Published: Elsevier 2014-12-01
• Series: Genomics Data
• Subjects: Nucleosome; MNase; Microarray; KSHV
• Online Access: http://www.sciencedirect.com/science/article/pii/S2213596014000397

In the eukaryotic nucleus, DNA is packaged into chromatin. The fundamental subunit of chromatin is the nucleosome, DNA is wrapped 1.6 times around a histone octamer core. Nuclear processes in eukaryotes are impacted by whether regulatory DNA is occupied by nucleosomes. We used microarrays to measure nucleosome occupancy in human cells post-Kaposi's sarcoma-associated herpesvirus (KSHV) reactivation at hundreds of immunity-related loci. The detailed analysis of these technologies can be found in recent publications from our lab [1,3]. We found that nucleosome redistributions displayed chromosome specific nucleosome occupancy. This resource can be used to map nucleosome distributions in a variety of biological contexts.