Improved reagent for trimethylsilylation of sphingolipid bases

• Main Authors: H.E. Carter, R.C. Gaver
• Format: Article
• Language: English
• Published: Elsevier 1967-07-01
• Series: Journal of Lipid Research
• Subjects: sphingolipid bases; gas-liquid chromatography; trimethylsilyl ether derivatives; separation; long-chain bases; erythro and threo isomers
• Online Access: http://www.sciencedirect.com/science/article/pii/S0022227520395717

This paper describes the trimethylsilylation of sphingolipid bases under conditions that give derivatives of improved stability. The retention times of the common C18 and C20 long-chain bases, including the anhydro bases, obtained on a commercially available gas-liquid chromatographic column with a nonpolar stationary phase are given. Data are also presented on the separation of the erythro and threo isomers of sphingosine and dihydrosphingosine, as the trimethylsilyl ethers of the N-acetyl derivatives.