Preparation and Characterization of Vancomycin-Loaded Electrospun Rana chensinensis Skin Collagen/Poly(L-lactide) Nanofibers for Drug Delivery

Collagen was extracted from abandoned Rana chensinensis skin in northeastern China via an acid enzymatic extraction method for the use of drug carriers. In this paper we demonstrated two different nanofiber-vancomycin (VCM) systems, that is, VCM blended nanofibers and core-shell nanofibers with VCM...

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Main Authors: Mei Zhang, Ziqi Li, Lihua Liu, Zhouyang Sun, Wendi Ma, Zhichao Zhang, Rui Zhang, Dahui Sun
Format: Article
Language:English
Published: Hindawi Limited 2016-01-01
Series:Journal of Nanomaterials
Online Access:http://dx.doi.org/10.1155/2016/9159364
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spelling doaj-a900e83c3f2a41fc8ec0b766447debf62020-11-25T00:10:17ZengHindawi LimitedJournal of Nanomaterials1687-41101687-41292016-01-01201610.1155/2016/91593649159364Preparation and Characterization of Vancomycin-Loaded Electrospun Rana chensinensis Skin Collagen/Poly(L-lactide) Nanofibers for Drug DeliveryMei Zhang0Ziqi Li1Lihua Liu2Zhouyang Sun3Wendi Ma4Zhichao Zhang5Rui Zhang6Dahui Sun7Alan G. MacDiarmid Laboratory, College of Chemistry, Jilin University, Changchun 130012, ChinaAlan G. MacDiarmid Laboratory, College of Chemistry, Jilin University, Changchun 130012, ChinaCollege of Quartermaster Technology, Jilin University, Changchun 130062, ChinaCollege of Quartermaster Technology, Jilin University, Changchun 130062, ChinaCollege of Quartermaster Technology, Jilin University, Changchun 130062, ChinaCollege of Quartermaster Technology, Jilin University, Changchun 130062, ChinaCollege of Quartermaster Technology, Jilin University, Changchun 130062, ChinaNorman Bethune First Hospital, Jilin University, Changchun 130021, ChinaCollagen was extracted from abandoned Rana chensinensis skin in northeastern China via an acid enzymatic extraction method for the use of drug carriers. In this paper we demonstrated two different nanofiber-vancomycin (VCM) systems, that is, VCM blended nanofibers and core-shell nanofibers with VCM in the core. Rana chensinensis skin collagen (RCSC) and poly(L-lactide) (PLLA) (3 : 7) were blended in 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) at a concentration of 10% (g/mL) to fabricate coaxial and blend nanofibers, respectively. Coaxial and blend electrospun RCSC/PLLA nanofibers containing VCM (5 wt%) were evaluated for the local and temporal delivery of VCM. The nanofiber scaffolds were characterized by environmental scanning electron microscope (ESEM), transmission electron microscopy (TEM), Fourier transform infrared spectra (FTIR), differential scanning calorimeter (DSC), water contact angle (WCA), and mechanical tests. The drug release of VCM in these two systems was compared by using UV spectrophotometer. The empirical result indicated that both the blend and coaxial RCSC/PLLA scaffolds followed sustained control release for a period of 80 hours, but the coaxial nanofiber might be a potential drug delivery material for its better mechanical properties and sustained release effect.http://dx.doi.org/10.1155/2016/9159364
collection DOAJ
language English
format Article
sources DOAJ
author Mei Zhang
Ziqi Li
Lihua Liu
Zhouyang Sun
Wendi Ma
Zhichao Zhang
Rui Zhang
Dahui Sun
spellingShingle Mei Zhang
Ziqi Li
Lihua Liu
Zhouyang Sun
Wendi Ma
Zhichao Zhang
Rui Zhang
Dahui Sun
Preparation and Characterization of Vancomycin-Loaded Electrospun Rana chensinensis Skin Collagen/Poly(L-lactide) Nanofibers for Drug Delivery
Journal of Nanomaterials
author_facet Mei Zhang
Ziqi Li
Lihua Liu
Zhouyang Sun
Wendi Ma
Zhichao Zhang
Rui Zhang
Dahui Sun
author_sort Mei Zhang
title Preparation and Characterization of Vancomycin-Loaded Electrospun Rana chensinensis Skin Collagen/Poly(L-lactide) Nanofibers for Drug Delivery
title_short Preparation and Characterization of Vancomycin-Loaded Electrospun Rana chensinensis Skin Collagen/Poly(L-lactide) Nanofibers for Drug Delivery
title_full Preparation and Characterization of Vancomycin-Loaded Electrospun Rana chensinensis Skin Collagen/Poly(L-lactide) Nanofibers for Drug Delivery
title_fullStr Preparation and Characterization of Vancomycin-Loaded Electrospun Rana chensinensis Skin Collagen/Poly(L-lactide) Nanofibers for Drug Delivery
title_full_unstemmed Preparation and Characterization of Vancomycin-Loaded Electrospun Rana chensinensis Skin Collagen/Poly(L-lactide) Nanofibers for Drug Delivery
title_sort preparation and characterization of vancomycin-loaded electrospun rana chensinensis skin collagen/poly(l-lactide) nanofibers for drug delivery
publisher Hindawi Limited
series Journal of Nanomaterials
issn 1687-4110
1687-4129
publishDate 2016-01-01
description Collagen was extracted from abandoned Rana chensinensis skin in northeastern China via an acid enzymatic extraction method for the use of drug carriers. In this paper we demonstrated two different nanofiber-vancomycin (VCM) systems, that is, VCM blended nanofibers and core-shell nanofibers with VCM in the core. Rana chensinensis skin collagen (RCSC) and poly(L-lactide) (PLLA) (3 : 7) were blended in 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) at a concentration of 10% (g/mL) to fabricate coaxial and blend nanofibers, respectively. Coaxial and blend electrospun RCSC/PLLA nanofibers containing VCM (5 wt%) were evaluated for the local and temporal delivery of VCM. The nanofiber scaffolds were characterized by environmental scanning electron microscope (ESEM), transmission electron microscopy (TEM), Fourier transform infrared spectra (FTIR), differential scanning calorimeter (DSC), water contact angle (WCA), and mechanical tests. The drug release of VCM in these two systems was compared by using UV spectrophotometer. The empirical result indicated that both the blend and coaxial RCSC/PLLA scaffolds followed sustained control release for a period of 80 hours, but the coaxial nanofiber might be a potential drug delivery material for its better mechanical properties and sustained release effect.
url http://dx.doi.org/10.1155/2016/9159364
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